16-202A

Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, FITC conjugate

clone JBW301, Upstate^®, from mouse

• 동의어(들): H2AXS139P, Histone H2A.X (phospho S139), H2A histone family, member X, H2AX histone
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 결합: FITC conjugate
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: JBW301, monoclonal
• 종 반응성: human
• 제조업체/상표: Upstate^®
• 기술: flow cytometry: suitable; immunocytochemistry: suitable
• 동형: IgG1
• NCBI 수납 번호: NM_002105.2
• UniProt 수납 번호: P16104
• 배송 상태: dry ice
• 타겟 번역 후 변형: phosphorylation (pSer139)
• 유전자 정보: human ... H2AX(3014)

설명

일반 설명

Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

특이성

Broad species cross-reactivity is expected based on conservation of sequence homology.

Recognizes Histone H2A.X phosphorylated at Ser139, MW 15 kDa.

면역원

Epitope: Ser139

KLH-conjugated, synthetic peptide (CKATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X. The immunizing sequence has 8 identical amino acids in yeast and mouse. Clone JBW301.

애플리케이션

Immunocytochemistry: 2-4 μg/mL of a previous lot detected phospho-histone H2A.X in etoposide-treated HeLa cells fixed with 95% ethanol/5% acetic acid (10 minutes), followed by 1% formaldehyde, 0.25% Triton^™ X-100 in TBS (5 min.).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Use Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, FITC conjugate (mouse monoclonal antibody) validated in FC, ICC to detect phospho-Histone H2A.X (Ser139) also known as H2AXS139P, H2AX histone.

품질

Evaluated by Flow Cytometry to detect Log phase Jurkat cells treated with staurosporine.

Flow Cytometry: Log phase Jurkat cells were treated with staurosporine (1 μg/mL) for the indicated time. Histone H2A.X (Ser 139) phosphorylation was detected as described in the manual for the H2A.X Phosphorylation Assay Kit (Flow Cytometry), Catalog # 17-344. Cells were analyzed on a Becton-Dickinson FACS-Calibur flow cytometer.

표적 설명

15 kDa

물리적 형태

Protein G Purified

Purified FITC-conjugated mouse monoclonal IgG1 in buffer containing PBS with 0.05% sodium azide. Frozen solution.

저장 및 안정성

Stable for 1 year at from date of receipt.

분석 메모

Control
Staurosporine-treated Jurkat cells

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable