204885

Complement C3, Human

Complement C3, Human, CAS 80295-41-6, is a native C3 component. Glycoprotein composed of two non-identical disulfide-bonded subunits of 115 kDa (α) and 75 kDa (β).

• 동의어(들): Complement C3, Human
• CAS Number: 80295-41-6
• MDL number: MFCD00130836
• UNSPSC 코드: 12352202
• NACRES: NA.25

속성

• Quality Level: 100
• 분석: ≥85% (SDS-PAGE)
• 형태: liquid
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; avoid repeated freeze/thaw cycles
• 불순물: IgG, IgA, IgM, C4, functionally active C5, albumin or ceruloplasmin, trace
• 배송 상태: wet ice
• 저장 온도: −70°C

설명

일반 설명

Native, human C3 complement component. Glycoprotein composed of two non-identical disulfide-bonded subunits with M.W. of 115 kDa (α) and 75 kDa (β). Present in normal human serum at 1.25 mg/ml. Classical and alternative activation pathways of complement converge at C3 step. Activation via either pathway can result in assembly of C3-cleaving enzymes (C3 convertases) on target surfaces. Both C3 convertases cleave the C3 α-chain at peptide bond 77 resulting in production of C3a (M.W. 9083) and C3b fragments (M.W. 180,000). Released C3a peptide is one of the three complement anaphylatoxins. The nascent C3b fragment can form a covalent ester bond with target surface. This covalent attachment of C3b to target acceptors is required for continuation of complement activation.

Native, human C3 complement component. Glycoprotein composed of two non-identical disulfide-bonded subunits with M.W. of 115,000 (α) and 75,000 (β). Present in normal human serum at 1.25 mg/ml. Classical and alternative activation pathways of complement converge at the C3 step. Activation via either pathway can result in assembly of C3-cleaving enzymes (C3 convertases) on target surfaces. Both C3 convertases cleave the C3 α-chain at peptide bond 77, resulting in production of C3a (M.W. 9083) and C3b fragments (M.W. 180,000). Released C3a peptide is one of the three complement anaphylatoxins. The nascent C3b fragment can form a covalent ester bond with target surface. The covalent attachment of C3b to target acceptors is required for continuation of complement activation.

생화학적/생리학적 작용

>3,000 C₃H₅0 units/mg; ≥60% of the C3 activity in normal human serum on a mg/mg basis.

경고

Toxicity: Standard Handling (A)

물리적 형태

In PBS.

제조 메모

Prepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

재구성

Following initial thaw, aliquot and freeze (-70°C).

기타 정보

Lambris, J.D. and Muller-Eberhard, H.J. 1986. Mol. Immunol.23, 1237.
Tack, B.F., et al. 1980. Methods Enzymol. 80, 64.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable