565758

β-Secretase Substrate IV, Fluorogenic

≥95% (HPLC), lyophilized solid, β-secretase substrate, Calbiochem^®

• 동의어(들): β-Secretase Substrate IV, Fluorogenic, FS-1, H-RE(EDANS)EVNLDAEFK(DABCYL)R-OH, Memapsin 2 Substrate I, Fluorogenic
• 실험식(Hill 표기법): C₉₁H₁₂₉N₂₅O₂₅S
• CAS Number: 310427-94-2
• Molecular Weight: 2005.22
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• product name: β-Secretase Substrate IV, Fluorogenic, A highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate, based on the EVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein (APP).
• Quality Level: 100
• 분석: ≥95% (HPLC)
• 형태: lyophilized solid
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; protect from light
• 색상: red
• solubility: DMSO: 5 mg/mL
• 형광: λ_ex ~350 nm; λ_em ~490 nm
• 배송 상태: ambient
• 저장 온도: −20°C

설명

일반 설명

A highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate (excitation max.: ~350 nm; emission max.: ~490), based on the GVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein. Hydrolysis of the substrate at Leu-Asp bond by β-secretase results in fluorescence enhancement. Reported K_cat/k_m values (min^-1/µM) for pro-mempasin 2 and its proteolytically activated species Leu^28p-mempasin 2 are 0.044 and 0.040, respectively (at pH 4.5, 37°C).

A highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate, based on the EVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein (APP). Hydrolysis of the substrate (Leu-Asp cleavage) by β-secretase results in fluorescence enhancement. k_cat/K_m values (min^-1/µM) at 37°C, pH 4.5, for pro-memapsin 2 and its activated species Leu^28p-memapsin 2 are 0.044 and 0.040, respectively.

생화학적/생리학적 작용

Cell permeable: no

Primary Target
β-secretase substrate

Product does not compete with ATP.

Reversible: no

포장

Packaged under inert gas

경고

Toxicity: Standard Handling (A)

서열

H-Arg-Glu(EDANS)Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(DABCYL)Arg-OH

물리적 형태

Supplied as a trifluoroacetate salt.

재구성

Following reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 1 month at -20°C.

기타 정보

Ermolieff, J., et al. 2000. Biochemistry 39, 12450.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable