648313

TRIS Hydrochloride

ULTROL^® Grade, ≥99% (titration), useful in pH range 7.0-9.0

• 동의어(들): Trizma^® hydrochloride, tris(Hydroxymethyl)aminomethane, HCl, TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride
• Linear Formula: NH₂C(CH₂OH)₃ · HCl
• CAS Number: 1185-53-1
• Molecular Weight: 157.60
• Beilstein: 3675235
• MDL number: MFCD00012590
• UNSPSC 코드: 12161705
• NACRES: NA.25

속성

• Quality Level: 100
• 분석: ≥99% (titration)
• 형태: solid
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze
• 색상: white
• 유용한 pH 범위: 7.0-9.0
• pKa(25 °C): 8.1
• solubility: water: 500 mg/mL
• 양이온 미량물: heavy metals: ≤5 ppm
• 배송 상태: ambient
• 저장 온도: 15-25°C
• SMILES string: Cl.NC(CO)(CO)CO
• InChI: 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
• InChI key: QKNYBSVHEMOAJP-UHFFFAOYSA-N

설명

일반 설명

Absorbance (1.0 M, H₂O, 260 nm):≤0.1.

Useful in the pH range of 7.0-9.0. Has a pK_a of 8.1 at 25°C.

Widely used as a biological buffer (absorbance: <0.1 at 260 nm, 1.0 M, H₂O). A 1.0 M solution in H₂O has a pH range of 3.5-5.0 and a pK_a of 8.3 at 20°C.

애플리케이션

• Effect of Organic Solvents on the Activity, Stability and Secondary Structure of asclepain cI, Using FTIR and Molecular Dynamics Simulations.: This study uses Tris hydrochloride as a buffer to assess the stability and structural changes in the asclepain cI enzyme, providing insights into enzyme applications in industrial and medical biotechnology (Origone et al., 2024).


• Microencapsulation of Omeprazole by Lactobacillus acidophilus ATCC 4356 Surface Layer Protein and Evaluation of its Stability in Acidic Condition.: Research demonstrates the use of Tris hydrochloride buffer in stabilizing microencapsulated omeprazole, aimed at enhancing drug delivery in acidic environments (Mobarak Qamsari et al., 2020).

경고

Toxicity: Standard Handling (A)

기타 정보

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

ULTROL is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable