추천 제품
제품명
Anti-Beta-Amyloid 1-42 Antibody, Chemicon®, from rabbit
생물학적 소스
rabbit
Quality Level
항체 형태
affinity purified immunoglobulin
항체 생산 유형
primary antibodies
클론
polyclonal
정제법
affinity chromatography
종 반응성
chicken, mouse, human
제조업체/상표
Chemicon®
기술
ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... APP(351)
일반 설명
The cerebral and vascular plaques associated with Alzheimer′s disease (AD) are mainly composed of amyloid beta peptides (Ab). Ab is derived from cleavage of the amyloid precursor protein (APP) and varies in length from 39 to 43 amino acids. Ab [1-40], Ab [1-42], and Ab [1-43] peptides result from cleavage of APP after residues 40, 42, and 43, respectively. The cleavage takes place by gamma-secretase during the last APP processing step. Ab [1-40], [1-42] and [1-43] peptides are major constituents of the plaques and tangles that occur in AD. Ab antibodies and peptides have been developed as tools for elucidating the biology of AD.
특이성
Recognizes beta-amyloid 1-42. Antibody does not show appreciable reactivity with beta amyloid 1-40 as detected by ELISA.
One of the most important and initial steps which causes loss of memory and cognition in Alzheimer’s Disease (AD) involves proteolytic cleavage of amyloid precursor protein (APP, chromosome 21) releasing short 40, 42 & 43 amino acid peptides (beta amyloid 1-40, 1-42 and 1-43). Polymerization of beta-amyloid and subsequent neuronal deposit (amyloid) leads to the degeneration of neurons involved in memory and cognition.
One of the most important and initial steps which causes loss of memory and cognition in Alzheimer’s Disease (AD) involves proteolytic cleavage of amyloid precursor protein (APP, chromosome 21) releasing short 40, 42 & 43 amino acid peptides (beta amyloid 1-40, 1-42 and 1-43). Polymerization of beta-amyloid and subsequent neuronal deposit (amyloid) leads to the degeneration of neurons involved in memory and cognition.
면역원
A six amino acid peptide sequence from the C-terminal of human beta-amyloid 1-42.
Epitope: C-terminus
애플리케이션
Anti-Beta-Amyloid 1-42 Antibody is an antibody against Beta-Amyloid 1-42 for use in ELISA, IH, IH(P) & WB.
Immunohistochemistry (Paraffin) Analysis:
Western Blot:
1-10 µg/mL of a previous lot was used. (Chemiluminescence technique).
ELISA:
1:10,000-1:100,000 of a previous lot was used using 50-100 ng immunogen peptide/well.
Optimal working dilutions must be determined by the end user.
Western Blot:
1-10 µg/mL of a previous lot was used. (Chemiluminescence technique).
ELISA:
1:10,000-1:100,000 of a previous lot was used using 50-100 ng immunogen peptide/well.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
품질
Routinely evaluated by immunohistochemistry (paraffin) on Alzheimer’s Disease-Brain.
Immunohistochemistry (Paraffin) Analysis:
Beta-Amyloid (cat. # AB5078P) staining on Alzheimer’s Disease-Brain. Tissue pretreated with Citrate, pH 6.0. The antibody was diluted to 1:35, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen as staining on plaque deposits (brown) or as cerebral vascular amyloid deposition in the walls of blood vessels (brown).
Immunohistochemistry (Paraffin) Analysis:
Beta-Amyloid (cat. # AB5078P) staining on Alzheimer’s Disease-Brain. Tissue pretreated with Citrate, pH 6.0. The antibody was diluted to 1:35, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen as staining on plaque deposits (brown) or as cerebral vascular amyloid deposition in the walls of blood vessels (brown).
물리적 형태
ImmunoAffinity Purified
Purified rabbit polyclonal in liquid buffer containing PBS with 0.1% BSA.
저장 및 안정성
Stable for 1 year at -20ºC from date of receipt.
분석 메모
Control
Alzheimer′s disease brain tissue, whole tissue extracts from mouse brain. C03-801.
Alzheimer′s disease brain tissue, whole tissue extracts from mouse brain. C03-801.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Accumulation of intraneuronal ?-amyloid 42 peptides is associated with early changes in microtubule-associated protein 2 in neurites and synapses.
Takahashi, RH; Capetillo-Zarate, E; Lin, MT; Milner, TA; Gouras, GK
Testing null
Ramon Velazquez et al.
Molecular neurodegeneration, 11(1), 52-52 (2016-07-15)
Alzheimer's disease (AD) is the most prevalent neurodegenerative disorder worldwide. Clinically, AD is characterized by impairments of memory and cognitive functions. Accumulation of amyloid-β (Aβ) and neurofibrillary tangles are the prominent neuropathologies in patients with AD. Strong evidence indicates that
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Mhatre, M; Hensley, K; Nguyen, A; Grammas, P
Journal of Neuroscience Research null
Constantinos D Paspalas et al.
Alzheimer's & dementia : the journal of the Alzheimer's Association, 14(5), 680-691 (2017-12-16)
An animal model of late-onset Alzheimer's disease is needed to research what causes degeneration in the absence of dominant genetic insults and why the association cortex is particularly vulnerable to degeneration. We studied the progression of tau and amyloid cortical
Matthew M Harper et al.
Investigative ophthalmology & visual science, 60(7), 2716-2725 (2019-06-28)
Traumatic brain injury (TBI) is a risk factor for developing chronic neurodegenerative conditions including Alzheimer's disease (AD). The purpose of this study was to examine chronic effects of blast TBI on retinal ganglion cells (RGC), optic nerve, and brain amyloid
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