추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
affinity isolated antibody
항체 생산 유형
primary antibodies
클론
polyclonal
정제법
affinity chromatography
종 반응성
human
종 반응성(상동성에 의해 예측)
rat (based on 100% sequence homology), Xenopus (based on 100% sequence homology), bovine (based on 100% sequence homology), chicken (based on 100% sequence homology), mouse (based on 100% sequence homology), porcine (based on 100% sequence homology)
기술
inhibition assay: suitable (peptide)
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
phosphorylation (pThr119)
유전자 정보
human ... BECN1(8678)
일반 설명
The Beclin-1 (BECN1) gene encodes a 60 kDa coiled-coil protein that interacts with the prototypic apoptosis inhibitor Bcl-2. Beclin-1 promotes autophagy in autophagy-defective yeast with a targeted disruption of agp6/vps30, and in human MCF7 breast carcinoma cells. The autophagy-promoting activity of Beclin-1 in MCF7 cells is associated with inhibition of MCF7 cellular proliferation, in vitro clonigenicity, tumorigenesis, and has been found to ameliorate the neurodegenerative pathology in α-Synuclein models of Parkinson′s and lewy body diseases. Endogenous Beclin-1 protein expression is frequently low in human breast epithelial carcinoma cell lines and tissue, but is expressed ubiquitously at high levels in normal breast epithelia. It has also been shown that Beclin 1 contains a leucine-rich nuclear export signal motif raising the possibility that its autophagy and/or tumor suppressor function may require regulated, CRM1-dependent, nucleocytoplasmic transport.
특이성
This antibody recognizes the BH3 domain of Beclin phosphorylated at Thr119.
면역원
Epitope: BH3 domain
KLH-conjugated linear peptide corresponding to the BH3 domain of human Beclin phosphorylated at Thr119.
애플리케이션
Detect Beclin using this rabbit polyclonal antibody, Anti-phospho Beclin Antibody (Thr119) validated for use in western blotting & Peptide Inhibition Assay.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
Western Blotting/Peptide Inhibition Analysis: 5 µg/mL from a representative lot detected phospho Beclin (Thr119) in LNCap cell lysate. Peptide inhibition was observed with the addition of phospho peptides, but not with the addition of non-phospho peptides.
품질
Evaluated by Western Blotting in human prostate tumor tissue lysate.
Western Blotting Analysis: 5 µg/mL of this antibody detected 10 µg of human prostate tumor tissue lysate.
Western Blotting Analysis: 5 µg/mL of this antibody detected 10 µg of human prostate tumor tissue lysate.
표적 설명
~50 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
물리적 형태
Affinity purified
Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
저장 및 안정성
Stable for 1 year at 2-8°C from date of receipt.
분석 메모
Control
Human prostate tumor tissue lysate.
Human prostate tumor tissue lysate.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Miray Turk et al.
Molecules (Basel, Switzerland), 26(4) (2021-02-11)
Expression levels of the major mammalian autophagy regulator Beclin 1 and its interaction with Bcl-2 regulate the switch between autophagic cell survival and apoptotic cell death pathways. However, some of the regulators and the precise mechanisms of these processes still
Changxuan Chen et al.
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas, 51(6), e7080-e7080 (2018-04-26)
Prostate cancer (PCa) is the second leading cause of cancer death in men. Irradiation is one of the available options for treatment of PCa, however, approximately 10-45% of PCa are resistant to irradiation. We aimed to explore the role of
M-L Plissonnier et al.
Oncogene, 36(48), 6712-6724 (2017-08-08)
Hepatitis C virus (HCV) infection is a leading cause of hepatocellular carcinoma (HCC), mainly through cirrhosis induction, spurring research for a deeper understanding of HCV versus host interactions in cirrhosis. The present study investigated crosstalks between HCV infection and UNC5A
David Mondaca-Ruff et al.
Frontiers in pharmacology, 9, 1553-1553 (2019-02-26)
Hypertension is a disease associated to increased plasma levels of angiotensin II (Ang II). Ang II can regulate proliferation, migration, ROS production and hypertrophy of vascular smooth muscle cells (VSMCs). However, the mechanisms by which Ang II can affect VSMCs
Hanan Abu-Tayeh Suleiman et al.
International journal of molecular sciences, 23(20) (2022-10-28)
The kidney glomerular filtration barrier (GFB) is enriched with heparan sulfate (HS) proteoglycans, which contribute to its permselectivity. The endoglycosidase heparanase cleaves HS and hence appears to be involved in the pathogenesis of kidney injury and glomerulonephritis. We have recently
문서
Autophagy is a regulated process involved in cell growth, development, and recycling of cytoplasmic components in cells.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
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