ABD116

Anti-Human Oct-4 Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): POU domain,class 5, transcription factor 1, NF-A3, Octamer-binding protein 3, Oct-3, Octamer-binding protein 4, Oct-4, Octamer-binding transcription factor 3, OTF-3
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.75

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human
• 종 반응성(상동성에 의해 예측): chimpanzee (based on 100% sequence homology), goat (based on 100% sequence homology)
• 기술: immunocytochemistry: suitable; western blot: suitable
• NCBI 수납 번호: NP_002692
• UniProt 수납 번호: Q01860
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... POU5F1(5460)

설명

일반 설명

Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.

특이성

Reacts with human Oct-4 only, and detects the Oct-4A isoform. (Note: Please order Catalog No. MAB4419 for use with mouse embryonic stem cells).

The clone reacts strongly with specific nuclear binding to human ES cells and cross-reacts weakly with mouse ES cells.

면역원

Epitope: unknown

KLH-EDC-conjugated linear peptide corresponding to the unknown of Human Oct-4.

애플리케이션

Immunocytochemistry Analysis: A 1:1000 dilution from a representative lot detected human Oct-4 in 10 µg of H9 human ES cells.

Research Category
Stem Cell Research

Stem Cell Research

Research Sub Category
Neural Stem Cells

Developmental Signaling

This Anti-Human Oct-4 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of Human Oct-4.

품질

Evaluated by Western Blotting in H9 human ES cell lysate and mouse ES cells lysate.

Western Blotting Analysis: A 0.25 µg/mL dilution of this antibody detected human Oct-4 in 10 µg of H9 human ES cell lysate but not in 10 µg of mouse ES cells lysate.

표적 설명

~40 kDa observed

물리적 형태

Antigen Affinity Purified

Purified rabbit Polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable