EZRGRT

Rat/Mouse Ghrelin ELISA Kit

measures and quantifies total Ghrelin in 20 μL serum or plasma

• 동의어(들): Appetite Regulating Hormone, Growth hormone-releasing peptide, Interleukin-6, Motilin-related peptide
• UNSPSC 코드: 12161503
• eCl@ss: 32161000
• NACRES: NA.32

속성

• product name: Rat/Mouse Ghrelin (total) ELISA, This Rat/Mouse Ghrelin (total) ELISA is used to measure & quantify Ghrelin (total) levels in Metabolism & Endocrine research.
• Quality Level: 200
• 종 반응성: mouse, rat
• 포장: kit of 1 × 96 wells
• 파라미터: 20 μL sample volume (4hr assay)
• assay range: accuracy: 80-103%; (Serum: Active Ghrelin); accuracy: 86-102%; (Plasma: Active Ghrelin); accuracy: 92-103%; (Serum: Des-octanoyl Ghrelin); accuracy: 95-107%; (Plasma: Des-octanoyl Ghrelin); inter-assay cv: 1.8-4.5%; intra-assay cv: 0.7-1.3%; (Serum); inter-assay cv: 2.0-3.2%; intra-assay cv: 1.1-1.7%; (Plasma)
• 기술: ELISA: suitable
• 입력: sample type plasma (K2 EDTA); sample type serum
• UniProt 수납 번호: Q9EQX0 (mouse); Q9QYH7 (rat); Q9UBU3
• 응용 분야: research use
• 검출 방법: colorimetric (450nm/590nm)
• 배송 상태: wet ice
• 저장 온도: 2-8°C

설명

일반 설명

Circulating ghrelin is a multifunctional hormone secreted primarily by the stomach. Originally found as an endogenous ligand for the growth hormone secretagogue receptor, it is also an appetite-stimulating hormone in the regulation of energy homeostasis. It consists of 28 amino acids and the n-octanoylation of serine-3 position is necessary for its bioactivity. The acylated form is extremely unstable in the blood; consequently, the predominant form is des-octanoyl ghrelin. Long considered a key player in obesity, ghrelin may also play a role in maintaining the balance between insulin and glucose and the development of diabetes. The spike in insulin secretion occurring after eating usually correlates with a dip in ghrelin production. Researchers determined that in rat models of uncontrolled diabetes, food intake still suppressed ghrelin concentrations in the blood, but only approximately half as effectively as when insulin was present.

특이성

Canine Ghrelin (Active) - 71%
Human Ghrelin (Active) - 115%
Human Des-Octanoyl Ghrelin - 249%
Porcine Ghrelin (Active) - 48%
Rat/Mouse Ghrelin (Active) - 85%
Rat/Mouse Des-Octanoyl Ghrelin - 100%

애플리케이션

Research Category
Metabolism

Research Sub Category
Obesity

Metabolic Disorders

Room temperature, 3 hour assay, 20 μL sample size (serum or plasma)

Used to detect/quantify: Ghrelin (Total)

기타 정보

Please contact Technical Service for linearity of dilution.

면책조항

For research use only. Not for use in diagnostic procedures.

안전성 정보

• 픽토그램: GHS06,GHS05,GHS09
• 신호어: Danger
• 유해 및 위험 성명서: H290,H302 + H332,H311,H315,H317,H319,H335,H411
• 예방조치 성명서: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects