추천 제품
제품명
Anti-Tubulin Antibody, beta III isoform, CT, clone TU-20 (Similar to TUJ1), ascites fluid, clone TU-20 (Similar to TUJ1), Chemicon®
생물학적 소스
mouse
Quality Level
항체 형태
ascites fluid
항체 생산 유형
primary antibodies
클론
TU-20 (Similar to TUJ1), monoclonal
종 반응성
rat, mouse, avian, human, bovine, monkey, pig, sheep
제조업체/상표
Chemicon®
기술
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable
동형
IgG1
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... TUBB3(10381)
일반 설명
Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins which makes up microtubules. Each has a molecular weight of approximately 55 kDa. Microtubules are assembled from dimers of α- and β-tubulin. These subunits are slightly acidic with an isoelectric point between 5.2 and 5.8. Tubulin was long thought to be specific to eukaryotes. Recently, however, the prokaryotic cell division protein FtsZ was shown to be evolutionarily related to tubulin.
특이성
Expected to cross-react with Avian, Porcine (Pig), Rat, Bovine, Sheep, Human, Monkey. Reactivity with other species has not been confirmed.
The antibody reacts with the C-terminus of the neuron specific beta III isoform of tubulin. Does not identify beta tubulin found in glial cells.
면역원
A synthetic peptide corresponding to amino acids 443-450 (ESEAQGPK) of human class III beta-tubulin conjugated to KLH.
Epitope: C-terminus
애플리케이션
Anti-Tubulin Antibody, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) is an antibody against Tubulin for use in ELISA, IC, IH, IH(P), IP & WB with more than 55 product citations.
Immunohistochemistry:
On formalin fixed frozen or paraffin embedded tissue sections: 1:20 to 1:100 dilution from a previous lot. High temperature citrate buffer antigen retrieval is recommended with paraffin embedded tissue sections.
Immunoprecipitation:
A previous lot of this antibody was used on Immunoprecipitation.
Immunocytochemistry:
1:100 to 1:500 dilution from a previous lot was used on fixed cells.
Immunoblotting:
1:1000. Antibody will detect a single band at 50 kDa corresponding to class III beta-tubulin.
ELISA:
A previous lot of this antibody was used on ELISA.
Optimal working dilution must be determined by the end user.
On formalin fixed frozen or paraffin embedded tissue sections: 1:20 to 1:100 dilution from a previous lot. High temperature citrate buffer antigen retrieval is recommended with paraffin embedded tissue sections.
Immunoprecipitation:
A previous lot of this antibody was used on Immunoprecipitation.
Immunocytochemistry:
1:100 to 1:500 dilution from a previous lot was used on fixed cells.
Immunoblotting:
1:1000. Antibody will detect a single band at 50 kDa corresponding to class III beta-tubulin.
ELISA:
A previous lot of this antibody was used on ELISA.
Optimal working dilution must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neuronal & Glial Markers
품질
Routinely evaluated by Western Blot on Mouse Brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected tubulin (βIII) on 10 μg of Mouse Brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected tubulin (βIII) on 10 μg of Mouse Brain lysates.
표적 설명
55 kDa
결합
Replaces: 04-1049
물리적 형태
Mouse monoclonal IgG1 ascites in buffer containing PBS containing 1% BSA and 15 mM sodium azide.
Unpurified
저장 및 안정성
Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
분석 메모
Control
Positive control: Brain tissue or neurons of any type.
Negative control: Non neural tissue or Astrocytes.
Positive control: Brain tissue or neurons of any type.
Negative control: Non neural tissue or Astrocytes.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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문서
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프로토콜
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