AB144P

Anti-Choline Acetyltransferase Antibody

CHEMICON^®, goat polyclonal

• 동의어(들): Choline acetylase, acetyl CoA:choline O-acetyltransferase, choline acetyltransferase, ChAT
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• product name: Anti-Choline Acetyltransferase Antibody, Chemicon^®, from goat
• 생물학적 소스: goat
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human, opossum, guinea pig, rat, mouse, zebrafish (THE JOURNAL OF COMPARATIVE NEUROLOGY 474:75–107 (2004).), avian, monkey, chicken
• 제조업체/상표: Chemicon^®
• 기술: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• NCBI 수납 번호: NM_020549.3
• UniProt 수납 번호: P28329
• 배송 상태: dry ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: chicken ... Chat(395314) ; human ... CHAT(1103) ; mouse ... Chat(12647) ; rat ... Chat(290567) ; rhesus monkey ... Chat(709977)

설명

일반 설명

Choline acetyltransferase (ChAT) has a molecular weight of about 68 kDa. It is synthesized in the parakaryon and is transported to the axon terminals. ChAT belongs to the choline/carnitine acyltransferase family. It seems that only cholinergic neurons have significant amounts of ChAT making anti-choline acetyltransferase a useful specific marker. Choline acetyltransferase is found in cholinergic neurons in brain and spinal cord.

특이성

Expected to cross-react with Avian, chicken, guinea pig, monkey, rat, and opposum.

면역원

Human placental enzyme.

애플리케이션

Anti-Choline Acetyltransferase Antibody has been used in:

• immunohistochemical staining
• western blotting
• immunocytochemistry

Immunohistochemistry:
A previous lot of this antibody was used at 1:100.

Optimal working dilutions and protocols must be determined by end user.

생화학적/생리학적 작용

Choline acetyltransferase (CHAT) is involved in the synthesis of acetylcholine in neurons. It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. CHAT catalyzes the transfer of an acetyl group between acetyl-coenzyme A and choline. Mutations in the gene encoding this protein have been associated with congenital neuromuscular disorders.

품질

Routinely evaluated by Western Blot on mouse brain lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected CHAT on 10 μg of mouse brain lysates.

표적 설명

70/74 kDa

물리적 형태

Affinity purified
Purified goat polyclonal in buffer containing 5 mg/mL BSA and 0.2% sodium azide.

저장 및 안정성

Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt. Avoid repeated freeze/thaw cycles.
For short term storage (3-4 weeks) maintain at 2-8°C.

분석 메모

Control
Human placenta lysates, rat forebrain/rostral hypothalamus.

기타 정보

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Research Category:
Neuroscience

Research Sub Category:
Neurotransmitters & Receptors

Neuronal & Glial Markers

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable