ZRB1012

Anti-Choline Acetyltransferase (ChAT) Antibody, clone 2K16, ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 동의어(들): CHOACTase, ChAT, Choline acetylase, EC: 2.3.1.6
• UNSPSC 코드: 12352203
• NACRES: NA.43

속성

• 생물학적 소스: rabbit (recombinant)
• Quality Level: 200
• 재조합: expressed in HEK 293 cells
• 결합: unconjugated
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 2K16, recombinant monoclonal; monoclonal
• 제품 라인: ZooMAb^® learn more
• 형태: lyophilized
• 분자량: calculated mol wt 82.54 kDa; observed mol wt ~65 kDa
• 종 반응성: rat, mouse
• 종 반응성(상동성에 의해 예측): human
• 포장: antibody small pack of 25 μL
• 환경친화적 대안 제품 특성: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 향상된 검증: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 기술: immunofluorescence: suitable using 1:100; immunohistochemistry: suitable using 1:100; western blot: suitable using 1:1,000
• 동형: IgG
• UniProt 수납 번호: P28329
• 환경친화적 대안 카테고리: Aligned,
• 배송 상태: ambient
• 저장 온도: 2-8°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... CHAT(1103)

설명

일반 설명

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

특이성

Clone 2K16 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Choline Acetyltransferase. It targets an epitope with in 19 amino acids from the N-terminal half.

면역원

KLH-conjugated linear peptide corresponding to 19 amino acids from the N-terminal half of human Choline acetyltransferase.

애플리케이션

Anti-Choline Acetyltransferase (ChAT), clone 2K16 ZooMAb, Cat. No. ZRB1012, is a recombinant Rabbit Monoclonal Antibody that specifically targets Choline Acetyltransferase (aka ChAT) and has been tested for use in Immunofluorescence, Immunohistochemistry, and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Choline Acetyltransferase (ChAT) in mouse brain tissue lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Choline Acetyltransferase (ChAT) in rat brain tissue sections.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Choline Acetyltransferase (ChAT) in mouse brain tissue sections.

표적 설명

Choline O-acetyltransferase (UniProt: P28329; also known as EC: 2.3.1.6, CHOACTase, ChAT, Choline acetylase) is encoded by the CHAT gene (Gene ID: 1103) in human. Choline acetyltransferase a single-strand globular protein that catalyzes the biosynthesis of acetylcholine from acetyl CoA and choline. It is one of the most specific indicator for monitoring the functional state of cholinergic neurons in the central and peripheral nervous systems. It is synthesized in the perikaryon of cholinergic neurons and transported to the nerve terminals. Mutations in CHAT gene are known to cause Myasthenic syndrome that is characterized by failure of neuromuscular transmission, which include pre-synaptic, synaptic, and post-synaptic disorders. Three isoforms of Choline acetyltransferase have been described that are produced by alternative splicing. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Oda, Y et al., (1999). Pathol. Int. 49(11); 921-937).

물리적 형태

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

재구성

300 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

법적 정보

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable