MAB16985
Anti-MCAM Antibody, clone P1H12
clone P1H12, Chemicon®, from mouse
동의어(들):
CD146 antigen, Cell surface glycoprotein P1H12, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen, melanoma adhesion molecule, melanoma cell adhesion molecule
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모든 사진(3)
About This Item
UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41
추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
P1H12, monoclonal
종 반응성
mouse, canine, human
반응하면 안 됨
rat
포장
antibody small pack of 25 μg
제조업체/상표
Chemicon®
기술
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
동형
IgG1
적합성
not suitable for immunohistochemistry (Paraffin)
NCBI 수납 번호
UniProt 수납 번호
배송 상태
ambient
저장 온도
2-8°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... MCAM(4162)
관련 카테고리
일반 설명
MUC18 antigen (CD146), a member of the immuoglobulin superfamily, is an intrinsic membrane glycoprotein of 110-120 kDa found on the surface of endothelial cells, bone marrow fibroblasts and various melanomas. MUC18 has been used as a marker of tumor progression in human melanoma because expression in those tumors correlates strongly with poor prognosis and the development of metastic disease. In addition, a number of human T, B and myeloid leukemic cell lines seem to express MUC18. The close structural relationship with N-CAM and related molecules suggests that MUC18 may be also a developmentally regulated cell adhesion molecule (Melanoma adhesion molecule or MCAM).
특이성
Detects circulating endothelial cells in human. Expected to cross-react with dog & mouse.
면역원
Immunization of mice with HUVEC.
애플리케이션
Anti-MCAM Antibody, clone P1H12 detects level of MCAM & has been published & validated for use in ELISA, FC, IC, IH, IP & WB.
Immunocytochemistry:
1-10 μg/mL of a previous lot worked in immunocytochemistry.Works best on EDTA or Trypsin lifted endothelial cells.
Immunohistochemistry:
1-10 µg/mL. 4% PFA for 30min RT or <2hrs at 4°C. Block w/ 1%BSA/0.2% tween20/PBS for 30min. Works well in frozen tissue; fixed or unfixed.
Immunoprecipitation:
1-10 μg/mL of a previous lot worked in immunoprecipitation.
ELISA:
1-10 μg/mL of a previous lot worked in ELISA.
FACS Analysis:
1-10 μg/mL of a previous lot worked in FACS.
Optimal working dilutions must be determined by end user.
1-10 μg/mL of a previous lot worked in immunocytochemistry.Works best on EDTA or Trypsin lifted endothelial cells.
Immunohistochemistry:
1-10 µg/mL. 4% PFA for 30min RT or <2hrs at 4°C. Block w/ 1%BSA/0.2% tween20/PBS for 30min. Works well in frozen tissue; fixed or unfixed.
Immunoprecipitation:
1-10 μg/mL of a previous lot worked in immunoprecipitation.
ELISA:
1-10 μg/mL of a previous lot worked in ELISA.
FACS Analysis:
1-10 μg/mL of a previous lot worked in FACS.
Optimal working dilutions must be determined by end user.
품질
Routinely evaluated by Western Blot on HUVEC lysates.
Western Blot Analysis:
1:500 dilution of this lot detected MCAM on 10μg of HUVEC lysates.
Western Blot Analysis:
1:500 dilution of this lot detected MCAM on 10μg of HUVEC lysates.
표적 설명
110 kDa
물리적 형태
Format: Purified
Purified mouse monoclonal IgG1 in buffer containing 0.02 M PB with 0.25 M NaCl, pH=7.6, with 0.1% sodium azide.
분석 메모
Control
HUVEC cells.
HUVEC cells.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Keiich Hirono et al.
Journal of the American College of Cardiology, 48(6), 1257-1264 (2006-09-19)
This study investigated patients with acute Kawasaki disease (KD) to validate myeloid-related protein (MRP)-8/MRP-14 as a marker of disease activity and severity of coronary artery lesion development. Both MRP-8 and -14, which are S100-proteins secreted by activated neutrophils and monocytes
Feng Li et al.
Bone, 47(3), 546-555 (2010-06-24)
Currently, there are conflicting data in literature regarding contribution of bone marrow stromal cells (BMSCs) to bone formation when the cells are systemically delivered in recipient animals. To understand if BMSCs contribute to bone cell phenotype and bone formation in
Ivan Depasquale et al.
Cancer cell international, 6, 9-9 (2006-04-01)
Lovastatin and other statins may reduce the development of melanomas. The effects on melanoma cells and their ability to enhance angiogenesis in a co-culture system presented an opportunity to assess whether Lovastatin act on melanoma cells, HUVEC or both types
Yong Wook Park et al.
The American journal of pathology, 165(6), 2087-2098 (2004-12-08)
Thrombospondin 2 (TSP2), a matricellular protein with a primary role in modulating cell-matrix interactions, has been implicated in tissue repair and foreign body responses. Here we show that TSP2 has regulatory function in the chronic inflammatory lesions of rheumatoid arthritis.
Catherine Larochelle et al.
Brain : a journal of neurology, 135(Pt 10), 2906-2924 (2012-09-15)
In multiple sclerosis, encephalitogenic CD4(+) lymphocytes require adhesion molecules to accumulate into central nervous system inflammatory lesions. Using proteomic techniques, we identified expression of melanoma cell adhesion molecule (MCAM) on a subset of human effector memory CD4(+) lymphocytes and on
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