추천 제품
생물학적 소스
rat
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
YL1/2, monoclonal
종 반응성
mouse, human, rat
제조업체/상표
Chemicon®
기술
ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
radioimmunoassay: suitable
western blot: suitable
동형
IgG2a
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... TUBA1A(7846)
일반 설명
Tubulins, major components of the cellular cytoskeleton, form microtubules that are critical for cellular structure, migration, cell division and intracellular trafficking. There are at least three types of tubulins, alpha, beta and gamma. Each has a molecular weight of approximately 55 kDa. Microtubules are large structures (300 angstrom diameter) composed of alternating alpha and beta subunits. Compounds that block microtubule formation, such as Taxol and the vinca alkaloids, inhibit cell division and are effective anti-cancer agents.
특이성
MAB1864 recognizes the α subunit of tubulin-specificity binding to so called Tyr-Tubulin, which is produced by post-translational modification of tubulin. The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C-terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin.
면역원
Epitope: The epitope recognizes a linear sequence requiring an aromatic residue at the C-terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin).
Yeast tubulin
애플리케이션
Detect Tubulin using this Anti-Tubulin Antibody, clone YL1/2 validated for use in ELISA, IH, IP, RIA & WB.
Immunohistochemistry:
1:50-1:100 dilutoin from a previous lot was used on frozen sections.
ELISA:
1:100-1:1,000 dilution from a previousl lot was used.
Immunoprecipitation:
A previous lot of this antibody was used on Immunoprecipitation.
Radioimmunoassy:
A previous lot of this antibody was used on Radioimmuoassy.
Optimal working dilutions must be determined by end user.
1:50-1:100 dilutoin from a previous lot was used on frozen sections.
ELISA:
1:100-1:1,000 dilution from a previousl lot was used.
Immunoprecipitation:
A previous lot of this antibody was used on Immunoprecipitation.
Radioimmunoassy:
A previous lot of this antibody was used on Radioimmuoassy.
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
품질
Routinely evaluated by Western Blot on A431 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected TUBULIN on 10 μg of A431 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected TUBULIN on 10 μg of A431 lysates.
표적 설명
55 kDa
결합
Replaces: 04-1117
물리적 형태
Format: Purified
Protein A purified
Purified rat monoclonal IgG2a in buffer containing phosphate buffered saline (pH 7.4) with 0.09% sodium azide.
저장 및 안정성
Stable for 6 months at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
분석 메모
Control
All tissue
All tissue
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Peter T Ruane et al.
Scientific reports, 6, 27456-27456 (2016-06-09)
Microtubules and their associated proteins (MAPs) underpin the polarity of specialised cells. Adenomatous polyposis coli (APC) is one such MAP with a multifunctional agenda that requires precise intracellular localisations. Although APC has been found to associate with kinesin-2 subfamily members
LRRK2 kinase regulates synaptic morphology through distinct substrates at the presynaptic and postsynaptic compartments of the Drosophila neuromuscular junction.
Lee, S; Liu, HP; Lin, WY; Guo, H; Lu, B
The Journal of Neuroscience null
Integrin alpha 6 beta 4 promotes migration, invasion through Tiam1 upregulation, and subsequent Rac activation.
Cruz-Monserrate, Z; O'Connor, KL
Neoplasia null
The auto-inhibitory domain and ATP-independent microtubule-binding region of Kinesin heavy chain are major functional domains for transport in the Drosophila germline.
Williams, LS; Ganguly, S; Loiseau, P; Ng, BF; Palacios, IM
Development null
Fang Wang et al.
Biology of reproduction, 85(4), 702-713 (2011-06-24)
Preimplantation mouse embryos of many strains become arrested at the 2-cell stage if the osmolarity of culture medium that normally supports development to blastocysts is raised to approximately that of their normal physiological environment in the oviduct. Arrest can be
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