추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
M-KID2, monoclonal
종 반응성
human
제조업체/상표
Chemicon®
기술
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
radioimmunoassay: suitable
동형
IgG1
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... ITGA3(3675)
일반 설명
Integrins are a family of dimeric proteins that mediate cell-cell and extracellular matrix adhesion. Each VLA subtype (VLA-1, VLA-2, VL-3, VLA-4, VLA-5) is composed of a distinct alpha subunit noncovalently associated with a common beta subunit, as an alpha-beta dimer. Integrin VLA-3 (very late antigen, alpha-3/beta-1) is a cell surface receptor that binds to fibronectin, laminin, collagen type I and VI. VLA-3 is typically restricted in normal tissue but is widely expressed in many cultured tumor cells and correlates with the degree of invasiveness of malignant melanoma cells.
특이성
Recognizes an epitope related to the VLA-3 complex (alpha-3/beta-1 integrin). Specificity was determined by immunoprecipitation from a detergent extract of 35S-methionine labeled KJ29 cells.
면역원
Human renal carcinoma cell line (KJ29).
애플리케이션
Immunohistochemistry (works on Acetone-fixed paraffin-embedded tissues)
ELISA/RIA
Immunoprecipitation. When immunoprecipitated samples are run on SDS-PAGE under a) non-reducing conditions, two bands will be detected with an apparent molecular weight of 110 and 150kDa; and b) reducing (2% B-ME) conditions, a single molecular species of 130kDa is detected. Under reducing conditions both alpha3 and beta1 subunits run with the same apparent molecular weight.
Optimal working dilutions must be determined by end user.
Anti-Integrin α3β1 Antibody, clone M-KID2 detects level of Integrin α3β1 & has been published & validated for use in ELISA, IP, RIA, IC, IH(P).
물리적 형태
Format: Purified
Immunoglobulin purified by Protein A. Purified immunoglobulin in PBS and 0.05% sodium azide as preservative.
저장 및 안정성
Maintain at 2°-8°C in undiluted aliquots for up to 12 months from date of receipt.
분석 메모
Control
Cell line: KJ29 renal carcinoma adherent cells.
Tissue: Kidney; antibody reacts with basal layer podocytes and basement membrane of distal tubules.
Cell line: KJ29 renal carcinoma adherent cells.
Tissue: Kidney; antibody reacts with basal layer podocytes and basement membrane of distal tubules.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Y Nakamura et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 49(4), 439-444 (2001-03-22)
The tetra-membrane-spanning protein CD9 forms a complex with a membrane-anchored heparin binding epidermal growth factor-like growth factor (HB-EGF) and integrin alpha3beta1 in some human and monkey cell lines. We show here the immunohistochemical distribution of CD9, HB-EGF, and integrin alpha3beta1
Mehrnoosh Saghizadeh et al.
Experimental eye research, 129, 66-73 (2014-12-03)
Overexpression of c-met and suppression of matrix metalloproteinase-10 (MMP-10) and cathepsin F genes was previously shown to normalize wound healing, epithelial and stem cell marker patterns in organ-cultured human diabetic corneas. We now examined if gene therapy of limbal cells
T Takeuchi et al.
The Journal of clinical investigation, 92(6), 3008-3016 (1993-12-01)
Upregulation of integrin adhesive receptors has been implicated in various pathological conditions. We examined expression and function of integrin adhesive receptors on peripheral blood lymphocytes from patients with systemic lupus erythematosus (SLE), particularly those with the complication of vasculitis, and
Enhanced wound healing, kinase and stem cell marker expression in diabetic organ-cultured human corneas upon MMP-10 and cathepsin F gene silencing.
Saghizadeh, M; Epifantseva, I; Hemmati, DM; Ghiam, CA; Brunken, WJ; Ljubimov, AV
Investigative Ophthalmology & Visual Science null
Sara Brilha et al.
Frontiers in immunology, 9, 1348-1348 (2018-07-11)
Pulmonary tuberculosis (TB) is caused by inhalation of Mycobacterium tuberculosis, which damages the bronchial epithelial barrier to establish local infection. Matrix metalloproteinase-1 plays a crucial role in the immunopathology of TB, causing breakdown of type I collagen and cavitation, but
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