MAB2081

Anti-β-Catenin Antibody, clone 5H10

clone 5H10, Chemicon^®, from mouse

• 동의어(들): Anti-CTNNB, Anti-EVR7, Anti-MRD19, Anti-NEDSDV, Anti-armadillo
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 5H10, monoclonal
• 종 반응성: rat, human
• 제조업체/상표: Chemicon^®
• 기술: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 동형: IgG1
• NCBI 수납 번호: NM_001098209.1; NM_001098210.1; NM_001904.3
• UniProt 수납 번호: P35222
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... CTNNB1(1499) ; rat ... Ctnnb1(84353)

설명

일반 설명

The transmembrane, calcium dependent molecule E-cadherin is considered to be one of the key molecules in the formation of the intercellular junctional complex and establishment of polarity in epithelial cells. Catenins, the alpha-subunit (102 kDa), beta-subunit (88 kDa) and gamma-subunit (82 kDa), [reviewed in Takeichi, 1991] are a group of proteins that interact with the intercellular domain of E-cadherin, resulting in complexes of E-cadherin/beta-catenin/alpha-catenin or E-cadherin/gamma-catenin/alpha-Catenin (Hinck et al., 1994). Beta-catenin, the vertebrate homologue of Drosophila segment polarity gene armadillo, and key element of the Wnt signal transduction pathways, is important in embryogenesis, cell transformation, and intercellular adhesion including the adhesion, motility and metastasis of cancer cells. Thus, catenin protein expression, regulation and localization are potentially important markers to predict motility and invasiveness of epithelial neoplasms. The regions of both alpha- and beta-catenin, located on 5q21-22 and 3p21, have been shown to be involved in the development of certain tumors (see Jiang, 1996; Schlosshauer et al., 2002) and reduced expressions of both alpha- and beta-catenin have been described in various tumors including breast carcinoma (Hashizume et al, 1996). Besides adhesion functions, beta-catenin binds to the tumor suppressor gene APC. APC mutation disturbs the equilibrium and cytoplasmic levels of free beta-catenin level in the cell and may have a role in tumorigenesis.

특이성

Recognizes an epitope in the carboxy terminus, amino acids 758-771, of human beta-catenin.

면역원

Produced by immunization with recombinant human beta-catenin.

애플리케이션

Anti-β-Catenin Antibody, clone 5H10 is a Mouse Monoclonal Antibody for detection of β-Catenin also known as beta catenin & has been tested in ICC, IHC, IHC(P), IP & WB.

Immunohistochemistry: Low cross-linking fixatives are recommended. Histochoice fixative is effective. Formalin and PFA are effective, however may show weaker staining.

Immunohistochemistry on formalin fixed paraffin embedded tissues requires light fixations and citric acid/microwave antigen recovery for staining; In brief, sections of 2μm thickness were treated after deparaffinization with 0.3% hydrogen peroxide in methanol for 30 minutes to inhibit endogenous peroxidase and washed with TBS solution (Tris-buffered saline pH 7.6). To enhance antigen retrieval, sections were microwave-pretreated in 0.01 M citrate buffer solution at 750 W for a 3 minute-cycle period repeated three times; amplified detection methods are recommended.

Immunocytochemistry: Effective for A431 and HT1080 cell lines with acetone or methanol, or light PFA fixation.

Immunoblotting

Immunoprecipitation

Optimal working dilutions must be determined by end-user.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

표적 설명

94 kDa

결합

Replaces: 04-1002

물리적 형태

Format: Purified

Protein A Purified IgG in liquid at 1 mg/mL in 0.02M PB with 0.25M NaCl, pH 7.6, containing 0.1% sodium azide.

Protein A purified

저장 및 안정성

Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

분석 메모

Control
A431 and HT1080 cell line extracts

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable