MAB3067
Anti-Connexin 43 Antibody, clone 4E6.2
clone 4E6.2, Chemicon®, from mouse
동의어(들):
Gap junction 43 kDa heart protein, connexin 43, gap junction protein, alpha 1, 43kDa, gap junction protein, alpha 1, 43kDa (connexin 43), gap junction protein, alpha-like
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모든 사진(4)
About This Item
UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41
추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
4E6.2, monoclonal
종 반응성
mouse, rat, pig, canine
종 반응성(상동성에 의해 예측)
human
제조업체/상표
Chemicon®
기술
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
동형
IgG1
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... GJA1(2697)
일반 설명
Connexins are highly homologous proteins that assemble as hexamers (connexons) to form gap junctions, small channels which allow for the passage of ions and other small molecules up to 1,200 daltons between neighboring cells. Gap junctions play an important role in a variety of cellular processes including homeostasis, intercellular metabolic cooperation and synchronization, morphogenesis, cell differentiation, and growth control. The most broadly studied connexins are the 26, 32, 43 and 45 kDa isoforms. Connexin 43 is a 382 a.a. protein belonging to the alpha-type (Group II) subfamily of connexin proteins and possessing four transmembrane regions, with cytoplasmic amino and carboxyl terminals. It undergoes rapid turnover in the cell and its monomers may reside in the ER/Golgi network, forming a reservoir available for assembly upon degradation of existing connexin-43 channels. Connexin 43 has been detected in a variety of tissues including: atrial and ventricular myocytes, granulose cells, astrocytes, fibroblasts, leptomeningeal cells, brain, lung, smooth muscle, uterine muscle, kidney, lens, epithelium, cornea and testis. Connexin 43 also may have a role in communication between migrating cardiac neural crest cells.
특이성
Connexin-43. Recognizes proteins of molecular weight 43-47 kDa on Western blots of mouse brain lysate. Mouse connexin 43 sequence is identical in rat and bovine, and differs by one amino acid in pig. This sequence has no known homology with other connexins. Also effective for detection of Cx43 in mouse keratinocyte cells and on frozen sections of rat heart.
면역원
A synthetic peptide corresponding to positions 252-270 of the mouse connexin 43 sequence. This sequence is identical in rat and bovine.
Epitope: C-terminus
애플리케이션
Anti-Connexin 43 Antibody, clone 4E6.2 detects level of Connexin 43 & has been published & validated for use in ELISA, IC, IH & WB.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Adhesion (CAMs)
Adhesion (CAMs)
Western Blot (colorimetric detection):
1:1000-10,000 freshly prepared lysates, proteinase inhibitors are recommended. Recognizes proteins of molecular weight 43-47 kDa on Western blots of mouse brain lysate.
Immunocytochemistry:
A 1:50-1:250 dilution of a previous lot was used in immunocytochemistry.
Immunohistochemistry:
A previous lot was used to detect Connexin 43 expression in the keratinocytes of the middle and upper epidermal layers of frozen human skin tissue (Arita, Ken, et al. (2006). Am. J. Pathol. 169:416-423).
Immunohistochemistry:
A previous lot was used to detect Connexin 43 expression in human and rat heart tissue (1:500 dilution).
ELISA:
A previous lot of this antibody was used in ELISA.
Optimal working dilutions must be determined by end user.
1:1000-10,000 freshly prepared lysates, proteinase inhibitors are recommended. Recognizes proteins of molecular weight 43-47 kDa on Western blots of mouse brain lysate.
Immunocytochemistry:
A 1:50-1:250 dilution of a previous lot was used in immunocytochemistry.
Immunohistochemistry:
A previous lot was used to detect Connexin 43 expression in the keratinocytes of the middle and upper epidermal layers of frozen human skin tissue (Arita, Ken, et al. (2006). Am. J. Pathol. 169:416-423).
Immunohistochemistry:
A previous lot was used to detect Connexin 43 expression in human and rat heart tissue (1:500 dilution).
ELISA:
A previous lot of this antibody was used in ELISA.
Optimal working dilutions must be determined by end user.
품질
Evaluated by Western Blot on rat brain lysates.
Western Blot Analysis:
1:1000 dilution of this antibody detected Connexin 43 on 10 μg of rat brain lysates
Western Blot Analysis:
1:1000 dilution of this antibody detected Connexin 43 on 10 μg of rat brain lysates
표적 설명
43-47 kDa
물리적 형태
Format: Purified
Protein A purified
Purified mouse monoclonal IgG1 liquid in PBS containing sodium azide.
저장 및 안정성
Stable for 1 year at 2-8ºC in undiluted aliquots from date of receipt.
분석 메모
Control
Mouse brain tissue lysate
Mouse brain tissue lysate
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Effect of right ventricular versus biventricular pacing on electrical remodeling in the normal heart.
Saba, S; Mehdi, H; Mathier, MA; Islam, MZ; Salama, G; London, B
Circulation. Arrhythmia and Electrophysiology null
Rengasayee Veeraraghavan et al.
Pflugers Archiv : European journal of physiology, 468(10), 1651-1661 (2016-08-12)
It was recently demonstrated that cardiac sodium channels (Nav1.5) localized at the perinexus, an intercalated disc (ID) nanodomain associated with gap junctions (GJ), may contribute to electrical coupling between cardiac myocytes via an ephaptic mechanism. Impairment of ephaptic coupling by
Connexin 43 confers resistance to hydrogen peroxide-mediated apoptosis.
Giardina, SF; Mikami, M; Goubaeva, F; Yang, J
Biochemical and biophysical research communications null
Rengasayee Veeraraghavan et al.
eLife, 7 (2018-08-15)
Computational modeling indicates that cardiac conduction may involve ephaptic coupling - intercellular communication involving electrochemical signaling across narrow extracellular clefts between cardiomyocytes. We hypothesized that β1(SCN1B) -mediated adhesion scaffolds trans-activating NaV1.5 (SCN5A) channels within narrow (<30 nm) perinexal clefts adjacent
Farida Goubaeva et al.
Biochemical and biophysical research communications, 352(1), 97-103 (2006-11-17)
Connexin 43 (Cx43) is thought to be present largely in the plasma membrane and its function solely to provide low resistance electrical connection between myocytes. A recent report suggested the presence of Cx43 in the mitochondria as well. We confirmed
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