MAB386

Anti-Myelin Basic Protein Antibody, a.a. 82-87

culture supernatant, clone 12, Chemicon^®

• 동의어(들): MBP
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rat
• Quality Level: 100
• 항체 형태: culture supernatant
• 항체 생산 유형: primary antibodies
• 클론: 12, monoclonal
• 종 반응성: human, rat, sheep, mouse, rabbit, chicken, guinea pig, bovine, all
• 제조업체/상표: Chemicon^®
• 기술: ELISA: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; radioimmunoassay: suitable; western blot: suitable
• 동형: IgG2a
• NCBI 수납 번호: NM_001025094.1
• UniProt 수납 번호: P02686
• 배송 상태: dry ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... MBP(4155) ; mouse ... Mbp(17196) ; rat ... Mbp(24547)

설명

일반 설명

The two major components of CNS myelin are Myelin Basic Protein (MBP) and proteolipid protein (PLP), occuring in roughly equimolar proportions. There are many neurological disorders associated with deficiencies of myelin assembly and structure. For example Multiple Sclerosis is a human disease due to multiple factors but generally characterized by the degradation of the myelin sheath. MBP ususally refers to the 18.5 kDa isoform, which is one of the most abundant proteins of the myelin sheath of adult human and bovine CNS. Genetically, MBP is the product of differential splicing of a single mRNA transcript. In humans there are 4 isoforms, 21.5, 20.2, 18.5, and 17.2 kDa. Thus, MBP are present in multiple isoforms, part of a large family of developmentally expressed, translocatable, and highly post-translationally modifed proteins with multiple binding partners. Structurally, MBP displays a predominately unorganized form with greater than 75% in random coil. Other non-CNS related functions of myelin include insulin induction and glucagon release from pancreatic islets.

특이성

Reacts with MBP from all species tested including human, bovine, sheep, rabbit, mouse, rat, guinea pig and chicken.

Reacts with MBP from all species tested. It reacts weakly with peptides ending in the phe 91 where the 91-92 phe-phe bond is broken. Synthetic peptide 82-99 reacts very well as does intact MBP. Mapped to the region DENPVV.

면역원

Bovine myelin basic protein

Epitope: a.a. 82-87

애플리케이션

Anti-Myelin Basic Protein Antibody, a.a. 82-87 is an antibody against Myelin Basic Protein for use in ELISA, IC, IH, RIA & WB.

Immunohistochemistry:
A previous lot of this antibody was used on frozen sections.

ELISA/RIA:
1:1000 dilution of a previous lot of this antibody was used in ELISA/RIA.

Western Blotting:
Identifies an 18-20kDa band in westerns.

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Neuronal & Glial Markers

Neurochemistry & Neurotrophins

품질

Evaluated by Western Blot on Mouse brain lysates.

Western Blot Analysis:
1:500 dilution of antibody lot detected four isoforms of MBP on 10 µg of Mouse brain lysates.

표적 설명

18-20 kDa

물리적 형태

Liquid containing 0.1% sodium azide.

Unpurified

저장 및 안정성

Stable for 1 year at -20ºC from date of receipt.

분석 메모

Control
Brain tissue

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable