추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
ascites fluid
항체 생산 유형
primary antibodies
클론
2-2B, monoclonal
종 반응성
mouse, rat, vertebrates, human
제조업체/상표
Chemicon®
기술
immunocytochemistry: suitable
immunohistochemistry: suitable
radioimmunoassay: suitable
western blot: suitable
동형
IgM
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... NCAM1(4684)
일반 설명
Polysialic acid (PSA) is a long, linear homopolymer that is attached to the neural cell adhesion molecule (NCAM). It has the remarkable ability to down-regulate a wide variety of contact-dependent cell interactions. This activity is believed to stem from the ability of the charged polymer to occupy a large hydrated volume and thereby cause a direct physical hindrance of cell–cell contact. The regulated expression of PSA has been shown to promote a reduction in cell interactions that can create permissive conditions for changes in the structure of a variety of tissues.
The broadest expression of PSA occurs on precursor cells during early development and plays a role in promotion of the migration of these cells after cell division. In most cases, PSA expression is lost after the migration process is completed. However, the axons of neurons often retain PSA to bundle,sprout, and branch appropriately during axon path-finding.
The broadest expression of PSA occurs on precursor cells during early development and plays a role in promotion of the migration of these cells after cell division. In most cases, PSA expression is lost after the migration process is completed. However, the axons of neurons often retain PSA to bundle,sprout, and branch appropriately during axon path-finding.
특이성
Reacts with alpha 2-8 linked neuraminic acid (NeuAc-alpha 2-8) n with n >10. This polymer is usually termed polysialic acid (PSA). In vertebrates PSA is linked to neural cell adhesion molecule (NCAM, CD56), in bacteria it is associated with capsula of meningococcus strain group B.
The monoclonal works well on all species expressing PSA-NCAM including mouse.
면역원
Viable Meningococcus group B (strain 355).
애플리케이션
Detect Polysialic Acid-NCAM using this Anti-Polysialic Acid-NCAM Antibody, clone 2-2B validated for use in IC, IH, RIA & WB with more than 30 product citations.
Western Blotting: A 1:500-1:1,000 concentration of a previous lot was used in WB.
Immunocyto/histochemistry: A 1:200-1:400 dilution of a previous lot of this antibody worked on live and fixed cells, tissue sections either frozen or fixed with any kind of fixative. (Rougon et al., 1986; Theodosis et al., 1991, 1999). It is not recommend that the antibody be used on paraffin embedded tissue since it gives variable results.
Radioimmunoassay (RIA) :
A previous lot of this antibody was used in RIA. (Figarella-Branger et al., 1996)
Cell sorting and cell panning (Ben-Hur et al., 1998)
Optimal working dilutions must be determined by end user.
Immunocyto/histochemistry: A 1:200-1:400 dilution of a previous lot of this antibody worked on live and fixed cells, tissue sections either frozen or fixed with any kind of fixative. (Rougon et al., 1986; Theodosis et al., 1991, 1999). It is not recommend that the antibody be used on paraffin embedded tissue since it gives variable results.
Radioimmunoassay (RIA) :
A previous lot of this antibody was used in RIA. (Figarella-Branger et al., 1996)
Cell sorting and cell panning (Ben-Hur et al., 1998)
Optimal working dilutions must be determined by end user.
품질
Routinely evaluated by Immunohistochemistry on hippocampus.
Immunohistochemistry(paraffin) Analysis:
PSA-NCAM (cat. # MAB5324) representative staining pattern/morphology on human hippocampus. Tissue pretreated with EDTA pH 8, antigen retrieval. Antibody was diluted to 1:100, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen as plasma membrane staining in neurons.
Optimal Staining With Epitope Retrieval: Alzheimer’s Hippocampus
Immunohistochemistry(paraffin) Analysis:
PSA-NCAM (cat. # MAB5324) representative staining pattern/morphology on human hippocampus. Tissue pretreated with EDTA pH 8, antigen retrieval. Antibody was diluted to 1:100, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen as plasma membrane staining in neurons.
Optimal Staining With Epitope Retrieval: Alzheimer’s Hippocampus
표적 설명
150-300 kDa
물리적 형태
Mouse monoclonal IgM in buffer containing 50% glycerol, no preservatives.
저장 및 안정성
Stable for 1 year at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgM and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgM and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
분석 메모
Control
Positive control tissue: Young Rat Hippocampus, human hippocampus.
Positive control tissue: Young Rat Hippocampus, human hippocampus.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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Testing null
Polysialic acid expression is not necessary for motor neuron target selectivity.
Robinson, GA; Madison, RD
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PloS one, 6(9), e24874-e24874 (2011-10-01)
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