추천 제품
product name
Anti-Vesicular Glutamate Transporter 1 Antibody, clone 3C10.2, Chemicon®, from mouse
생물학적 소스
mouse
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
3C10.2, monoclonal
종 반응성
mouse
종 반응성(상동성에 의해 예측)
rat
제조업체/상표
Chemicon®
기술
immunohistochemistry: suitable
western blot: suitable
동형
IgG1
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
rat ... Slc17A7(116638)
일반 설명
VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate. Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate. Vesicular glutamate transporters pack the neurotransmitter into synaptic vesicles so that they can be released into the synapse. VGLUTs are dependent on a proton gradient that they create by hydrolysing adenosine triphosphate (ATP). VGLUTs have only between one hundredth and one thousandth the affinity for glutamate that EAATs have.
특이성
Reacts with VGLUT1 (Vesicular Glutamate Transporter 1).
면역원
Recombinant protein from rat VGLUT1.
애플리케이션
Immunohistochemistry:
A previous lot of this antibody was tested on IH.
Optimal working dilutions must be determined by end user.
A previous lot of this antibody was tested on IH.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Neuronal & Glial Markers
Ion Channels & Transporters
Neuronal & Glial Markers
This Anti-Vesicular Glutamate Transporter 1 Antibody, clone 3C10.2, Cat. No. MAB5502, is validated for use in IH, WB for the detection of VGluT1.
품질
Routinely evaluated by Western Blot on Mouse Brain lysates.
Western Blot Analysis:
1:500 dilution of this lot detected Vesicular Glutamate Transporter 1 on 10 μg of Mouse Brain lysates.
Western Blot Analysis:
1:500 dilution of this lot detected Vesicular Glutamate Transporter 1 on 10 μg of Mouse Brain lysates.
표적 설명
60 kDa
물리적 형태
Format: Purified
Protein A purified
Purified mouse monoclonal IgG1 in buffer containing PBS containing 0.1% sodium azide.
저장 및 안정성
Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.
분석 메모
Control
Brain
VGLUT1 Control Peptide AG208
Brain
VGLUT1 Control Peptide AG208
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
적합한 제품을 찾을 수 없으신가요?
당사의 제품 선택기 도구.을(를) 시도해 보세요.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
April P Neal et al.
Toxicological sciences : an official journal of the Society of Toxicology, 116(1), 249-263 (2010-04-09)
Lead (Pb(2+)) exposure is known to affect presynaptic neurotransmitter release in both in vivo and cell culture models. However, the precise mechanism by which Pb(2+) impairs neurotransmitter release remains unknown. In the current study, we show that Pb(2+) exposure during
Kajari Karmakar et al.
Cell reports, 18(1), 185-197 (2017-01-05)
Tonotopy is a hallmark of auditory pathways and provides the basis for sound discrimination. Little is known about the involvement of transcription factors in brainstem cochlear neurons orchestrating the tonotopic precision of pre-synaptic input. We found that in the absence
Mizuya Shinoyama et al.
Frontiers in cellular neuroscience, 7, 128-128 (2013-08-24)
Hypoxic-ischemic encephalopathy (HIE) at birth could cause cerebral palsy (CP), mental retardation, and epilepsy, which last throughout the individual's lifetime. However, few restorative treatments for ischemic tissue are currently available. Cell replacement therapy offers the potential to rescue brain damage
M M Hamza et al.
Molecular neurobiology, 53(8), 5203-5216 (2015-09-25)
The cerebellum is a structure of the central nervous system involved in balance, motor coordination, and voluntary movements. The elementary circuit implicated in the control of locomotion involves Purkinje cells, which receive excitatory inputs from parallel and climbing fibers, and
Pietro Fazzari et al.
eLife, 3 (2014-06-04)
Neuregulin 1 (NRG1) and the γ-secretase subunit APH1B have been previously implicated as genetic risk factors for schizophrenia and schizophrenia relevant deficits have been observed in rodent models with loss of function mutations in either gene. Here we show that
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.