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생물학적 소스
mouse
Quality Level
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
14E11.2, monoclonal
종 반응성
human, mouse
기술
immunocytochemistry: suitable
western blot: suitable
동형
IgG2bκ
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... RB1CC1(9821)
일반 설명
Focal adhesion kinase family interacting protein of 200 kDa (FIP200) is a novel protein inhibitor for focal adhesion kinase (FAK). FIP200 inhibits kinase activity and associated cellular functions, such as cell adhesion, spreading, and motility in fibroblasts by binding directly to FAK.
The previously assigned protein identifier Q86YR4 has been merged into Q8TDY2. Full details can be found on the UniProt database.
The previously assigned protein identifier Q86YR4 has been merged into Q8TDY2. Full details can be found on the UniProt database.
특이성
This antibody binds to the interaction domain of FIP200, which is needed for the formation of the ULK-FIP200 complex under starved, autophagy conditions.
면역원
Epitope: Interaction domain
GST-tagged recombinant protein corresponding to the interaction domain of human FIP200.
애플리케이션
Anti-FIP200 Antibody, clone 14E11.2 is an antibody against FIP200 for use in Western Blotting, ICC.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
Western Blot Analysis: 1 µg/mL from a representative lot detected FIP200 in serum starved and non-serum starved HeLa cell lysate.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected FIP200 in NIH/3T3, A431, and HeLa cells.
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected FIP200 in untreated and chloroquinone-treated HUVEC cells.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected FIP200 in NIH/3T3, A431, and HeLa cells.
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected FIP200 in untreated and chloroquinone-treated HUVEC cells.
품질
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected FIP200 in 10 µg of HeLa cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected FIP200 in 10 µg of HeLa cell lysate.
표적 설명
~200 kDa observed. An uncharacterized band may be observed at ~50 kDa in some cell lysates.
물리적 형태
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
저장 및 안정성
Stable for 1 year at 2-8°C from date of receipt.
분석 메모
Control
HeLa cell lysate
HeLa cell lysate
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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문서
Autophagy is a regulated process involved in cell growth, development, and recycling of cytoplasmic components in cells.
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