DUO92101

Duolink^® In Situ Red Starter Kit Mouse/Rabbit

• 동의어(들): in situ Proximity Ligation Assay, Protein Protein Interaction Assay
• UNSPSC 코드: 41116158
• NACRES: NA.32

속성

• 제품 라인: Duolink^®
• Quality Level: 100
• 기술: proximity ligation assay: suitable
• 형광: λ_ex 594 nm; λ_em 624 nm (Texas Red^®; Zeiss Filter set 31)
• 적합성: suitable for fluorescence
• 저장 온도: −20°C

설명

애플리케이션

Duolink^®proximity ligation assay(PLA^®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

Follow the Duolink^® In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.

Visit our Duolink^® PLA Resource Center for information on how to run a Duolink^® experiment, applications, troubleshooting, and more.

To perform a complete Duolink^® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes.This starter kit supplies all other necessary reagents for 30 Duolink^® PLA reactions, which include a pair of PLA probes (Anti-Rabbit PLUS and Anti-Mouse MINUS), red detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink^® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.

Specificity
The Duolink^® In Situ Red Starter Kit Mouse/Rabbit requires one primary antibody from mouse and one primary antibody from rabbit. Red fluorescence detection reagents are often used with Texas Red^® filter.

Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink^® PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink^® projects

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Duolink^® in situ red starter kit has been used in proximity ligation assay.

특징 및 장점

• No overexpression or genetic manipulation required
• High specificity (fewer false positives)
• Single molecule sensitivity due to rolling circle amplification
• Relative quantification possible
• No special equipment needed
• Quicker and simpler than FRET
• Increased accuracy compared to co-IP
• Publication-ready results

기타 정보

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

법적 정보

Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany

PLA is a registered trademark of Merck KGaA, Darmstadt, Germany

Texas Red is a registered trademark of Life Technologies

키트 구성품 역시 별도로 이용 가능함

• DUO92002 Duolink^® In Situ PLA^® Probe Anti-Rabbit PLUS, Affinity purified Donkey anti-Rabbit IgG (H+L)
• DUO92004 Duolink^® In Situ PLA^® Probe Anti-Mouse MINUS, Affinity purified Donkey anti-Mouse IgG (H+L)
• DUO92008 Duolink^® In Situ Detection Reagents Red
• DUO82049 Duolink^® In Situ Wash Buffers, Fluorescence
• DUO82040 Duolink^® In Situ Mounting Medium with DAPI

안전성 정보

• 픽토그램: GHS08,GHS05,GHS09
• 신호어: Danger
• 유해 및 위험 성명서: H290,H317,H334,H411
• 예방조치 성명서: P234 - P261 - P272 - P273 - P280 - P302 + P352
• Hazard Classifications: Aquatic Chronic 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 8A - Combustible corrosive hazardous materials