MABE912

Anti-Ikaros Antibody, clone 4E9

clone 4E9, from mouse

• 동의어(들): DNA-binding protein Ikaros, Ikaros family zinc finger protein 1, Lymphoid transcription factor LyF-1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 4E9, monoclonal
• 종 반응성: human, mouse
• 기술: ChIP: suitable (ChIP-seq); flow cytometry: suitable; western blot: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_001207694
• UniProt 수납 번호: Q13422
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... IKZF1(10320) ; mouse ... Ikzf1(22778)

설명

일반 설명

Ikaros, also known as IKZF1, is a transcription factor that is important for the regulation of lymphopoiesis and also the development of the immune system. It has also been linked to adult and fetal erythroid differentiation in humans. Ikaros has been shown to induce local chromatin accessibility during CD4+ T cell differentiation and therefore, activating Th2 expression.

면역원

Recombinant protein corresponding to Human Ikaros.

애플리케이션

Flow Cytometry Analsysis: 1 µg of a representative lot of this antibody detected Ikaros in Mouse thymocytes

Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody as part of a blend Ikaros clones E9 and Ik14 (catalogue number MABE912 and MABE913 respectively) was demonstrated to perform in ChIP. See Zhang et al. (2012).

ChIP-seq Analsysis: A representative lot of this antibody as part of a blend Ikaros clones E9 and Ik14 (catalogue number MABE912 and MABE913 respectively) was demonstrated to perform in ChIP-seq. See Zhang et al. (2012).

Research Category
Epigenetics & Nuclear Function

Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Chromatin Biology

This Anti-Ikaros Antibody, clone 4E9 is validated for use in Western Blotting and Flow Cytometry and Chromatin Immunoprecipitation (ChIP) and ChIP-seq for the detection of Ikaros.

품질

Evaluated by Western Blotting in a Jurkat cell lysate.

Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Ikaros in a Jurkat cell lysate.

표적 설명

~60 kDa observed. Uncharacterized bands may be observed in some lysates.

물리적 형태

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable