MABF227

Anti-Interferon Lambda 4, clone 4G1 Antibody

clone 4G1, 1 mg/mL, from mouse

• 동의어(들): Interferon lambda-4, IFN-lambda-4
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 4G1, monoclonal
• 종 반응성: nonhuman primates, human
• 반응하면 안 됨: porcine, giant panda, canine, bat, bovine
• 농도: 1 mg/mL
• 기술: ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_001263183
• UniProt 수납 번호: K9M1U5
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: bat ... Loc102244039(102244039) ; human ... IFNL4(101180976)

설명

일반 설명

Interferon Lambda 4, also known as IFN-lambda-4, and encoded by the gene IFNL4, is an important cytokine that forms part of our innate immunity against viruses. Interferon lambda-4 activates the JAK-STAT signaling pathway and ultimately up regulates several interferon specific stimulated genes. Interferon lambda-4 expression can be stimulated by nucleotide strands particularly ployinosinic-polycytidylic acid (polyI:C) which mimics the double-stranded hepatitis C virus. A genetic variation in the Interferon lambda-4 gene is associated with susceptibility to HCV infection. The mutation of a single base insertion results in gene inactivation resulting in defective immune response to HCV. Interferon lambda-4 signaling operates through Interferon lambda R1 and IL-10R2 receptors and the mutation is associated with defective glycosylation which is required for secretion, thus the amplification of the signal becomes compromised resulting in susceptibility to HCV as well as coronaviruses.

특이성

Clone 4G1 detected IFNL4 expression induction in primary hepatocytes (e.g. by PolyIC or by HCV) and exogenously overexpressed IFNL4 in HepG2 cells. IFNL4 expression is not detectable in normal liver cells (Dr. Ludmila Prokunina-Olsson, National Cancer Institute, NIH, Bethesda, MD). Clone 4G1 does not cross-react with IFN-alpha or IL-28B (Prokunina-Olsson, L., et al. (2013). Nat. Genet. 45(2):164-171).

면역원

Linear peptide corresponding to an internal sequence from the N-termninal half of human interferon lambda 4.

애플리케이션

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Interferon Lambda 4 in human and rat liver tissue sections.

ELISA Analysis: A representative lot was conjugated with a sulfo-tag (MSD) and employed as the detection antibody in a sandwich ELISA application. An exogenously expressed IFNL4 HaloTag fusion was found secreted in the culture media from IFNL4-Halo construct-transfected, but not mock-transfected HepG2 cells (Onabajo, O.O., et al. (2015). J. Interferon. Cytokine Res. 35(11):888-900).

Flow Cytometry Analysis: A representative lot immunostained HepG2 cells transfected to express HaloTag fusion IFN-λ4 orthologs of elephant, human and non-human primate (chimpanzee, orangutan, rhesus, marmoset, cynomolgus) origins, but not cells expressing IFNL4 of bovine, bat, canine, panda, and porcine species (Paquin, A., et al. (2016). J. Interferon Cytokine Res. 36(1):30-36).

Immunocytochemistry Analysis: Representative lots immunostained HepG2 cells transfected to express HaloTag fusion of IFN-λ4 orthologs of elephant, human and non-human primate (chimpanzee, orangutan, rhesus, marmoset, cynomolgus) origins (Paquin, A., et al. (2016). J. Interferon Cytokine Res. 36(1):30-36; Prokunina-Olsson, L., et al. (2013). Nat. Genet. 45(2):164-171).

Immunocytochemistry Analysis: A representative lot detected a time-dependent induction of IFNL4 expression in hepatocytes from a donor heterozygous for ss469415590 (TT/ΔG) upon PolyI:C treatment or hepatitis C virus (HCV) strain JFH1HCV infection in cultures (Prokunina-Olsson, L., et al. (2013). Nat. Genet. 45(2):164-171).

Western Blotting Analysis: A representative lot detected HaloTag fusion IFN-λ4 orthologs of elephant, human and non-human primate (chimpanzee, orangutan, rhesus, marmoset, cynomolgus) origins, but not IFNL4 of bovine, bat, canine, panda, and porcine species transiently expressed in HepG2 cells (Paquin, A., et al. (2016). J. Interferon Cytokine Res. 36(1):30-36).

Western Blotting Analysis: A representative lot detected exogenously expressed IFNL4 HaloTag fusion constructs in transfected COS-7 and HepG2 cells, but not in untransfected cells. Clone 4G1 does not cross-react with IFN-alpha or IL-28B (Prokunina-Olsson, L., et al. (2013). Nat. Genet. 45(2):164-171).

This Anti-Interferon Lambda 4 antibody, clone 4G1 is validated for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting for the detection of Interferon Lambda 4.

품질

Evaluated by Western Blotting of human IFNL4 recombinant protein.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected 0.1 µg of human IFNL4 recombinant protein.

표적 설명

~21 kDa observed.

물리적 형태

Format: Purified

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable