추천 제품
생물학적 소스
rat
Quality Level
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
Decma-1, monoclonal
종 반응성
human, mouse
기술
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
동형
IgG1κ
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... CDH1(999)
mouse ... Cdh1(12550)
관련 카테고리
일반 설명
Cadherins are a family of transmembrane glycoproteins involved in Ca2+-dependent cell to cell interactions. The members of the family are differentially expressed in various tissues and function in the maintenance of tissue integrity, morphogenesis, and migration. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin), neural cadherin (N-cadherin), placental cadherin (P-cadherin) and retinal cadherin (R-cadherin). Type II include kidney cadherin (K-cadherin) and osteoblast cadherin (OB-cadherin). One of the best characterized cadherins is E-cadherin (epithelial cadherin or cadherin-1). It consists of calcium-binding extracellular domains responsible for interactions with other E-cadherin molecules on neighboring cells and interaction with integrin alphaEbeta7. The cytoplasmic domain of E-cadherin is linked to the actin cytoskeleton through cytoplasmic catenin proteins, thus establishing a complex localized to adherens junctions. In carcinomas, E-cadherin is frequently downregulated, consistent with its function of an invasion suppressor in normal epithelia.
면역원
Mouse embryonal carcinoma cell line PCC4
애플리케이션
Immunohistochemistry Analysis: 1:300 dilution of this antibody has been shown to detect E-cadherin in mouse GI tract tissue.
Inhibition: A previous lot was used by an independent laboratory in inhibion studies, blocking E-cadherin associated cell adhesion. (Vestweber, 1985)
Flow Cytometry Analysis: A previous lot was used by an independent laboratory in flow cytometry applications. (Tang, 1994)
Inhibition: A previous lot was used by an independent laboratory in inhibion studies, blocking E-cadherin associated cell adhesion. (Vestweber, 1985)
Flow Cytometry Analysis: A previous lot was used by an independent laboratory in flow cytometry applications. (Tang, 1994)
Research Category
Cell Structure
Cell Structure
Research Sub Category
Adhesion (CAMs)
Adhesion (CAMs)
This Anti-E-cadherin Antibody, clone DECMA-1 is validated for use in WB, IH, IC, IP, FC, FUNC for the detection of E-cadherin.
품질
Evaluated by Western Blot in A431 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected E-Cadherin in 10 µg of A431 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected E-Cadherin in 10 µg of A431 cell lysate.
표적 설명
~ 106 kDa observed MW.
물리적 형태
Format: Purified
Protein G
Purified rat monoclonal IgG1κ in buffer containing PBS without preservative.
저장 및 안정성
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
분석 메모
Control
A431 cell lysate
A431 cell lysate
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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