추천 제품
형태
tablet
포장
pkg of 30 tablets (individually packed in foil blister packs)
제조업체/상표
Roche
농도
(Starting concentration: 1 tablet contains protease inhibitors sufficient for a 10 ml cell extract.)
기술
inhibition assay: suitable
solubility
aqueous buffer: soluble
water: soluble
흡수
0.08 at 280 nm
저장 온도
2-8°C
일반 설명
cOmplete, Mini, EDTA-free tablets, inhibit a broad spectrum of serine and cysteine proteases. In contrast to other cOmplete tablets they do not contain EDTA, leaving the stability and the function of metal-dependant proteins unaffected. The affinity purification of Poly-His-tagged fusion proteins using IMAC (Immobilized Metal Affinity Chromatography) is also facilitated (no dialysis necessary).
Due to the optimized composition of the tablets they show excellent inhibition of serine and cysteine proteases, and are well suited for the protection of proteins isolated from animal tissues, plants, yeast, and bacteria. cOmplete, Mini, EDTA-free tablets, contains both irreversible and reversible protease inhibitors. Metallo- and aspartic proteases are not inhibited.
cOmplete Mini, EDTA-free Tablets are identical to cOmplete Mini Tablets, the only difference being that no EDTA or other chelating (e.g. EGTA) agents are included.
Due to the optimized composition of the tablets they show excellent inhibition of serine and cysteine proteases, and are well suited for the protection of proteins isolated from animal tissues, plants, yeast, and bacteria. cOmplete, Mini, EDTA-free tablets, contains both irreversible and reversible protease inhibitors. Metallo- and aspartic proteases are not inhibited.
cOmplete Mini, EDTA-free Tablets are identical to cOmplete Mini Tablets, the only difference being that no EDTA or other chelating (e.g. EGTA) agents are included.
특이성
Mixture of several protease inhibitors. For the inhibition of serine, cysteine, but not metalloproteases.
애플리케이션
cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail has been used as a component of
- nonidet P-40 (NP-40) buffer for the preparation of cell lysate of neuroblastoma cells
- tissue lysis buffer for homogenisation and lysis of prefrontal cortex tissues
- ice-cold radioimmunoprecipitation assay (RIPA) buffer for the lysis of Chinese hamster ovary (CHO-K1) cells
cOmplete™, Mini, EDTA-free, is used for the inhibition of serine and cysteine proteases in bacterial, yeast, plant, and animal cell extracts.
cOmplete, Mini, EDTA-free Tablets, are used for the inhibition of proteolytic activity in small volumes (up to 10 ml) in which EDTA may interfere with protein stability (e.g., metal-containing proteins) or subsequent assays. Since EDTA interferes with IMAC (Immobilized Metal Affinity Chromatography), cOmplete, Mini, EDTA-free, is preferentially used in the isolation process of Poly-His-tagged fusion proteins.
If it is necessary to inhibit proteolytic activity in a large volume (up to 50 ml), we recommend to use cOmplete, EDTA-free.
cOmplete, Mini, EDTA-free Tablets, are used for the inhibition of proteolytic activity in small volumes (up to 10 ml) in which EDTA may interfere with protein stability (e.g., metal-containing proteins) or subsequent assays. Since EDTA interferes with IMAC (Immobilized Metal Affinity Chromatography), cOmplete, Mini, EDTA-free, is preferentially used in the isolation process of Poly-His-tagged fusion proteins.
If it is necessary to inhibit proteolytic activity in a large volume (up to 50 ml), we recommend to use cOmplete, EDTA-free.
특징 및 장점
- Convenience: Simply push the quick-dissolving tablet through the foil packaging of the EASYpack into your buffer.
- Complete protection: Instantly protect your proteins against a broad range of proteases (see Table 1).
- Flexible: Protect proteins in extracts from almost any tissue or cell, including animals, plants, yeast, bacteria, or fungi.
- Safe: Choose non-toxic inhibitors that pose no risk to you or those around you.
포장
30 individually packed cOmplete Protease Inhibitor Cocktail Tablets in foil blisters. Each tablet is sufficient for a volume of 10 ml solution.
품질
Performance-tested with pancreas extract.
물리적 형태
Tablets in foil blister packs
제조 메모
Working concentration: 1 tablet per 10 ml extraction solution
Working solution: Handling
Carefully push the tablet through the foil packaging using the base of your thumb (not fingernail) to prevent the breakage of tablets.
Preparation of Working Solution
One cOmplete Mini EDTA-free tablet is sufficient for the inhibition of the proteolytic activity in 10 ml extraction solution. When very high proteolytic activity is present, one tablet should be used for 7 ml extraction buffer. Tablets can be added directly to the extraction medium. Alternatively a stock solution (7 X conc.) can be prepared.
Stock solution (7 X conc.)
Dissolve one cOmplete Mini EDTA-free tablet in 1.5 ml dist. water or in 1.5 ml 100 mM phosphate buffer, pH 7.0.
Storage conditions (working solution): The stock solution can be stored at 2 to 8 °C for 1 to 2 weeks, or at least 12 weeks at -15 to -25 °C.
Working solution: Handling
Carefully push the tablet through the foil packaging using the base of your thumb (not fingernail) to prevent the breakage of tablets.
Preparation of Working Solution
One cOmplete Mini EDTA-free tablet is sufficient for the inhibition of the proteolytic activity in 10 ml extraction solution. When very high proteolytic activity is present, one tablet should be used for 7 ml extraction buffer. Tablets can be added directly to the extraction medium. Alternatively a stock solution (7 X conc.) can be prepared.
Stock solution (7 X conc.)
Dissolve one cOmplete Mini EDTA-free tablet in 1.5 ml dist. water or in 1.5 ml 100 mM phosphate buffer, pH 7.0.
Storage conditions (working solution): The stock solution can be stored at 2 to 8 °C for 1 to 2 weeks, or at least 12 weeks at -15 to -25 °C.
재구성
Soluble in aqueous buffers, or add directly to extraction media. Alternatively, prepare 7x stock solutions in 1.5 ml water or 100 mM phosphate buffer, pH 7.0. Stock solution is stable for 1-2 weeks at 2 to 8 °C, or at least 12 weeks at -15 to -25 °C. Can be used in thiol-containing solutions at 15 to 25 °C.
저장 및 안정성
Store at 2 to 8 °C. (storage at -15 to -25 °C is also possible)
기타 정보
For life science research only. Not for use in diagnostic procedures.
법적 정보
cOmplete is a trademark of Roche
신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point (°F)
does not flash
Flash Point (°C)
does not flash
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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관련 콘텐츠
Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.
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