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Merck
모든 사진(1)

문서

10104159001

Roche

DNase I

grade II, from bovine pancreas

동의어(들):

Deoxyribonuclease, Deoxyribonucleate 5′-oligonucleotido-hydrolase

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모든 사진(1)

About This Item

UNSPSC 코드:
12352204

생물학적 소스

bovine pancreas

Quality Level

100

형태

lyophilized

포장

pkg of 100 mg

제조업체/상표

Roche

기술

DNA extraction: suitable

최적 pH

~7.0

저장 온도

2-8°C

관련 카테고리

일반 설명

Bovine pancreatic deoxyribonuclease I (DNase I) is a DNA minor grove-interacting nuclease which shows relatively low specificity. This protein is composed of two central β sheets, each composed of six β-strands. This structure is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III. DNase I is one of the most well characterized endonucleases of mammalian origin. It is a double-strand-specific endonuclease that requires bivalent cations for maximal activity.

애플리케이션

Isolation procedures for proteins (e.g., membrane proteins).
Bovine pancreatic deoxyribonuclease I (DNase I) has been used for-
  • The isolation of cells from lung, skin and tumor samples
  • Bacterial DNA extraction from live bacterial cells that are resistant to DNase I †

단위 정의

One unit is the enzyme activity that causes an increase in the absorbance of 0.001 per minute under assay conditions.

제조 메모

Activator: Ca2+ (0.12 mM) in combination with Mg2+ enhances activity [Laskowski (1971); Melgar and Goldthwait (1968)].
Working concentration: 1 mg/ml
Do not vortex while dissolving!
Storage conditions (working solution): Reconstituted in water, the solution can be kept for 2 to 3 days at 2 to 8 °C. Do not vortex during dissolving. Note: Dissolve at least 1 mg/ml.

기타 정보

For life science research only. Not for use in diagnostic procedures.

픽토그램

Health hazard
GHS08

신호어

Danger

유해 및 위험 성명서

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

does not flash

Flash Point (°C)

does not flash

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

Alejandro A Pezzulo et al.
Applied and environmental microbiology, 79(19), 5936-5941 (2013-07-23)
Human lungs are constantly exposed to bacteria in the environment, yet the prevailing dogma is that healthy lungs are sterile. DNA sequencing-based studies of pulmonary bacterial diversity challenge this notion. However, DNA-based microbial analysis currently fails to distinguish between DNA
Mitsuteru Natsuizaka et al.
American journal of cancer research, 4(1), 29-41 (2014-02-01)
Insulin-like growth factor binding protein 3 (IGFBP3), a hypoxia-inducible gene, regulates a variety of cellular processes including cell proliferation, senescence, apoptosis and epithelial-mesenchymal transition (EMT). IGFBP3 has been linked to the pathogenesis of cancers. Most previous studies focus upon proapoptotic
M Salimi et al.
Clinical and experimental dermatology, 41(5), 552-556 (2016-01-26)
Studying skin immune cells under various pathophysiological conditions is vital for understanding the nature of cutaneous inflammatory responses. Available methods of isolating cells from the skin have relatively low yield or require in vitro culture. To increase the effective isolation
Yanhui Cai et al.
Journal of immunology (Baltimore, Md. : 1950), 192(6), 2821-2829 (2014-02-19)
Alveolar macrophages (AMs) obtained by bronchoalveolar lavage (BAL) are commonly used to study lung macrophage-mediated immune responses. Questions remain, however, about whether AMs fully represent macrophage function in the lung. This study was performed to determine the contribution of interstitial
Weiwei Sheng et al.
Cellular and molecular gastroenterology and hepatology, 10(2), 434-449 (2020-04-25)
Enterochromaffin-like (ECL) cells in the stomach express gastrin/cholecystokinin 2 receptor CCK2R and are known to expand under hypergastrinemia, but whether this results from expansion of existing ECL cells or increased production from progenitors has not been clarified. We used mice
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