10127655001

Glucose-6-Phosphate Dehydrogenase (G6P-DH)

grade I, from yeast

• 동의어(들): G6P-DH, Glucose-6-Phosephate Dehydrogenase
• 효소 위원회 번호: 1.1.1.49 (BRENDA, IUBMB)
• UNSPSC 코드: 12352204

속성

• 생물학적 소스: yeast
• Quality Level: 100
• 형태: suspension
• 특이 활성도: ~350 units/mg protein (At 25 °C with glucose-6-P as the substrate.)
• 포장: pkg of 1 mL (5 mg/ml)
• 제조업체/상표: Roche
• 농도: ≥0.1-1.0 % (w/w)
• 기술: activity assay: suitable
• 색상: white to yellowish
• 최적 pH: 9.2
• solubility: water: miscible
• 적합성: suitable for UV spectrophotometry and general use
• NCBI 수납 번호: CAA93357
• UniProt 수납 번호: P11412
• 응용 분야: life science and biopharma
• 외래 활성: 6-PGDH <0.0100%; CK <0.00100%; GR <0.0100%; HK <0.0100%; PGI <0.00200%; PGluM <0.0100%
• 배송 상태: wet ice
• 저장 온도: 2-8°C
• 유전자 정보: Saccharomyces cerevisiae ... ZWF1(855480)

설명

일반 설명

Glucose-6-phosphate dehydrogenase (G6P DH) has an active dimeric form, whereas its monomeric and tetrameric forms are inactive. At low NADP⁺ and NADPH levels and under normal conditions, the enzyme exists as equilibrium of tetramers and dimers, whereas at high NADP⁺ and NADPH levels the equilibrium shifts to inactive tetramers.

애플리케이션

Component of cofactor recycling systems for NADPH.

생화학적/생리학적 작용

Glucose-6-phosphate dehydrogenase (G6P-DH) is involved in the pentose phosphate pathway. It is responsible for catalysing the oxidation of glucose-6-phosphate (G6P) to gluconolactone-6-phosphate, with NADP⁺ simultaneously accepting hydrogen to form NADPH, thereby maintaining NADPH levels in cells.

규격

Contaminants: <0.001% CK, <0.01% GR, HK, 6-PGDH, and PGluM each, <0.002% PGI each, <0.002% PGI

Approximately 350U/mg at +25°C with glucose-6-phosphate as the substrate.

물리적 형태

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

제조 메모

Activator: Mg²⁺ (5 to 10 mM)

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash