10165875001

Glucose-6-Phosphate Dehydrogenase (G6P-DH)

from Leuconostoc mesenteroides

• 동의어(들): G6P-DH, Glucose-6-Phosephate Dehydrogenase
• 효소 위원회 번호: 1.1.1.49 (BRENDA, IUBMB)
• UNSPSC 코드: 12352204

속성

• 생물학적 소스: bacterial (Leuconostoc mesenteroides)
• Quality Level: 100
• 형태: solution; suspension
• 특이 활성도: ~550 units/mg protein (At 25 °C (650 U/mg at 30 °C) with glucose-6-P and NAD as the substrates.)
• 분자량: dimer 110 kDa
• 포장: pkg of 1 mL (1,000 U)
• 제조업체/상표: Roche
• 농도: ≥0.1-1.0 % (w/w)
• 기술: activity assay: suitable
• 색상: white
• 최적 pH: 7.0-8.5(maximal activity at 7.8)
• solubility: water: miscible
• NCBI 수납 번호: AAA25265
• UniProt 수납 번호: P11411
• 응용 분야: life science and biopharma
• 외래 활성: 6-PGDH <0.001%; CK <0.001%; GR <0.01%; HK <0.05%; NADH oxidase <0.02%; PGI <0.01%
• 배송 상태: wet ice
• 저장 온도: 2-8°C

설명

일반 설명

Glucose-6-Phosphate Dehydrogenase (G6PD) is a ubiquitous enzyme which acts as a catalyst in producing pentose. It also produces NADPH for various biosynthetic and detoxification reactions. Sometimes G6PD provides an alternative to main glycolytic pathway for glucose utilization. It also has its application as DNA markers on X-chromosome.1

특이성

Specificity: At pH 7.8, 25 °C: G6P-DH from Leuconostoc (LG6PDH) is highly specific for D-glucose-6-phosphate (K_m = 36 μM, NADP as coenzyme; 64 μM, NAD as coenzyme), but will use either NADP (K_m = 7.4 μM; relative rate 1.0) or NAD (K_m = 115 μM; relative rate 1.8) as coenzyme.
LG6P-DH does not react with fructose-6-phosphate, fructose-1,6-biphosphate, glucose-1-phosphate or ribose-1-phosphate. LG6P-DG will oxidize 2-deoxy-glucose-6-phosphate with NADP, but not with NAD as coenzyme. There is a slow reaction with D-glucose.
Heat inactivation: The ammonium sulfate suspension is not inactivated when heated to temperatures ≤ 50 °C for 10 minutes. At temperatures > 60 °C the enzyme is rapidly inactivated.

애플리케이션

Component of cofactor recycling systems for NADPH.

It was used in enzymatic determination of C6 phosphorylation of glycogen.

품질

Contaminants: <0.001% CK, <0.01% GR and PGI each, <0.02% “NADH oxidase”, <0.05% HK, <0.001% 6-PGDH

단위 정의

Unit Conversion: One unit (U) [+25 °C] ≈ 1.18 U [+30 °C] ≈ 1.45 U [+37 °C], all with NAD as coenzyme

Unit Definition: One unit (U) LG6P-DH oxidizes 1 mol of glucose-6-phosphate and reduces 1 mol of NAD in 1 minute at +25 °C and pH 7.8.

물리적 형태

Solution of 1,000 U in 1 ml 3.2 M ammonium sulfate, pH approximately 6

제조 메모

Activator: HCO^3- (≤ 0.3 M) activates slightly.

분석 메모

Absorbance of purified enzyme: 1.15 (1 mg enzyme/ml, 280.5 nm)

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash