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10127655001

Roche

Glucose-6-Phosphate Dehydrogenase (G6P-DH)

grade I, from yeast

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Synonym(s):
G6P-DH, Glucose-6-Phosephate Dehydrogenase
Enzyme Commission number:

biological source

yeast

Quality Level

form

suspension

specific activity

~350 units/mg protein (At 25 °C with glucose-6-P as the substrate.)

packaging

pkg of 1 mL (5 mg/ml)

manufacturer/tradename

Roche

concentration

≥0.1-1.0 % (w/w)

technique(s)

activity assay: suitable

color

white to yellowish

optimum pH

9.2

solubility

water: miscible

suitability

suitable for UV spectrophotometry and general use

NCBI accession no.

UniProt accession no.

application(s)

life science and biopharma

foreign activity

6-PGDH <0.0100%
CK <0.00100%
GR <0.0100%
HK <0.0100%
PGI <0.00200%
PGluM <0.0100%

shipped in

wet ice

storage temp.

2-8°C

Gene Information

Saccharomyces cerevisiae ... ZWF1(855480)

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G5885G8404G8529
Gene Information

Saccharomyces cerevisiae ... ZWF1(855480)

Gene Information

-

Gene Information

-

Gene Information

-

biological source

yeast

biological source

bacterial (Leuconostoc mesenteroides)

biological source

bacterial (Leuconostoc mesenteroides)

biological source

-

technique(s)

activity assay: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

technique(s)

-

concentration

≥0.1-1.0 % (w/w)

concentration

-

concentration

-

concentration

-

suitability

suitable for UV spectrophotometry and general use

suitability

-

suitability

-

suitability

-

General description

Glucose-6-phosphate dehydrogenase (G6P DH) has an active dimeric form, whereas its monomeric and tetrameric forms are inactive. At low NADP+ and NADPH levels and under normal conditions, the enzyme exists as equilibrium of tetramers and dimers, whereas at high NADP+ and NADPH levels the equilibrium shifts to inactive tetramers.

Application

Component of cofactor recycling systems for NADPH.

Biochem/physiol Actions

Glucose-6-phosphate dehydrogenase (G6P-DH) is involved in the pentose phosphate pathway. It is responsible for catalysing the oxidation of glucose-6-phosphate (G6P) to gluconolactone-6-phosphate, with NADP+ simultaneously accepting hydrogen to form NADPH, thereby maintaining NADPH levels in cells.

Specifications

Contaminants: <0.001% CK, <0.01% GR, HK, 6-PGDH, and PGluM each, <0.002% PGI each, <0.002% PGI
Approximately 350U/mg at +25°C with glucose-6-phosphate as the substrate.

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Preparation Note

Activator: Mg2+ (5 to 10 mM)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash


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