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G6PDHII-RO

Roche

Glucose-6-Phosphate Dehydrogenase (G6P-DH)

grade II, from yeast

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Synonym(s):
G6P-DH, Glucose-6-Phosephate Dehydrogenase
Enzyme Commission number:

biological source

yeast

Quality Level

form

suspension

specific activity

~140 units/mg protein (At 25 °C with glucose-6-P as the substrate; standardized with BSA.)

packaging

pkg of 2 mL (10127671001 [5 mg/mL])
pkg of 5 mL (10737232001 [5 mg/mL])

manufacturer/tradename

Roche

optimum pH

9.2

pH range

5-11

shipped in

wet ice

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This Item
10165875001HKG6PDH-RO10127655001
specific activity

~140 units/mg protein (At 25 °C with glucose-6-P as the substrate; standardized with BSA.)

specific activity

~550 units/mg protein (At 25 °C (650 U/mg at 30 °C) with glucose-6-P and NAD as the substrates.)

specific activity

-

specific activity

~350 units/mg protein (At 25 °C with glucose-6-P as the substrate.)

form

suspension

form

solution, suspension

form

suspension

form

suspension

optimum pH

9.2

optimum pH

7.0-8.5(maximal activity at 7.8)

optimum pH

-

optimum pH

9.2

shipped in

wet ice

shipped in

wet ice

shipped in

-

shipped in

wet ice

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

General description

Glucose-6-Phosphate Dehydrogenase (G6PD) is a ubiquitous enzyme which acts as a catalyst in producing pentose. It also produces NADPH for various biosynthetic and detoxification reactions. Sometimes G6PD provides an alternative to main glycolytic pathway for glucose utilization. It also has its application as DNA markers on X-chromosome.

Application

Glucose-6-Phosphate Dehydrogenase (G6P-DH) is a component of cofactor recycling systems for NADPH. Additional reagent glucose-6-phosphate.
It was used in enzymatic determination of C6 phosphorylation of glycogen.

Quality

Contaminants: <0.001% CK, <0.05% GR, <0.01% 6-PGDH and PGluM each, <0.02% HK, <0.002% PGI

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6.0

Preparation Note

Activator: Mg2+ (5 to 10 mM)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash


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Anna A DePaoli-Roach et al.
The Journal of biological chemistry, 290(2), 841-850 (2014-11-25)
Glycogen is a branched polymer of glucose that acts as an energy reserve in many cell types. Glycogen contains trace amounts of covalent phosphate, in the range of 1 phosphate per 500-2000 glucose residues depending on the source. The function

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