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P3397

Sigma-Aldrich

Phosphoglucomutase from rabbit muscle

ammonium sulfate suspension, ≥100 units/mg protein

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Synonym(s):
PGM, α-D-Glucose-1,6-bisphosphatase, α-D-Glucose-1-phosphate phosphotransferase
CAS Number:
Enzyme Commission number:
MDL number:
NACRES:
NA.54

biological source

rabbit muscle

description

ammonium sulfate suspension, >=100 units/mg protein

form

ammonium sulfate suspension

specific activity

≥100 units/mg protein

storage condition

(Tightly closed)

technique(s)

activity assay: suitable

color

white to light yellow

foreign activity

lactic dehydrogenase ≤0.5%
phosphoglucose isomerase ≤0.01%
pyruvate kinase ≤0.05%

shipped in

wet ice

storage temp.

2-8°C

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SRE0069G6751P6635
vibrant-m

P6635

Phosphorylase b from rabbit muscle

biological source

rabbit muscle

biological source

-

biological source

rabbit muscle

biological source

rabbit muscle

specific activity

≥100 units/mg protein

specific activity

≥100 units/mg protein

specific activity

100-300 units/mg protein

specific activity

≥20 units/mg protein

form

ammonium sulfate suspension

form

lyophilized powder

form

ammonium sulfate suspension

form

lyophilized powder

storage condition

(Tightly closed)

storage condition

-

storage condition

(Tightly closed)

storage condition

(Keep container tightly closed in a dry and well-ventilated place)

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

General description

Research area: Cell Signaling

Phosphoglucomutase (PGM), a conserved enzyme, is abundantly found in animals, plants, and microorganisms. It is distributed in almost all tissues.

Application

Phosphoglycomutase has been used:

  • to study glycogenesis and phosphoglycomutase deficiency in humans
  • in phosphoglucomutase assays to study metabolic regulation
  • for assay A to assay enzymes closely linked to glycogen metabolism in transformed BL21(DE3)C43 cells
  • in sucrose synthase (SuSy) andADPglucose pyrophosphorylase (AGPase) assays(3)
  • in enzyme activity assay for coupling the reduction of nicotinamide adenine dinucleotide phosphate (NADP+)to determine glucose-1-phosphate from sucrose and inorganic phosphate (Pi)

Biochem/physiol Actions

Phosphoglucomutase(PGM) enzyme plays a vital role in glycolysis and gluconeogenesis. It is involved in glycogen and trehalose metabolism in insects. PGM participates in the development of plants and some microorganisms. It is essential for proteins, lipids, and nucleic acid metabolism and is crucial for the development of plants because glucose-6-phosphate is a crucial central metabolite. PGM catalyzes the interconversion of glucose-6-phosphate (G-6-P)and glucose-1-phosphate (G-1-P).

Unit Definition

One unit will convert 1.0 μmole of α-D-glucose 1-phosphate to α-D-glucose 6-phosphate per min at pH 7.4 at 30 °C.

Physical form

Crystalline suspension in 3.2 M (NH4)2SO4, pH 6.0 containing 0.01% EDTA

Analysis Note

Protein determined by biuret.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Molecular cloning of a gene encoding the sucrose phosphorylase from Leuconostoc mesenteroides B-1149 and the expression in Escherichia coli
Lee Ha J, et al.
Enzyme and Microbial Technology, 39, 612-620 (2006)
M B Dworkin et al.
The Journal of biological chemistry, 262(35), 17038-17045 (1987-12-15)
When 32P-labeled phosphoenolpyruvate is injected into Xenopus laevis oocytes, a 50-60-kDa protein of subunit size Mr 29,000 is rapidly labeled, followed by a second (monomeric) protein of 66 kDa concomitant with the loss of label from the first protein. We
H Sugie et al.
Neurology, 38(4), 602-605 (1988-04-01)
We report a 5-month-old boy with recurrent vomiting, lethargy, and poor weight gain. He had profound metabolic acidosis and nonketotic dicarboxylic aciduria. The serum and muscle carnitine levels were significantly low (60% and 10% of the control means, respectively), suggesting
Nora Alonso-Casajús et al.
Journal of bacteriology, 188(14), 5266-5272 (2006-07-04)
To understand the biological function of bacterial glycogen phosphorylase (GlgP), we have produced and characterized Escherichia coli cells with null or altered glgP expression. glgP deletion mutants (DeltaglgP) totally lacked glycogen phosphorylase activity, indicating that all the enzymatic activity is
Leszek A Kleczkowski et al.
Plants (Basel, Switzerland), 11(12) (2022-06-24)
UDP-glucose pyrophosphorylase (UGPase) carries a freely reversible reaction, using glucose-1-P and UTP to produce UDP-glucose (UDPG) and pyrophosphate (PPi), with UDPG being essential for glycosylation reactions in all organisms including, e.g., synthesis of sucrose, cellulose and glycoproteins. In the present

Articles

Instructions for working with enzymes supplied as ammonium sulfate suspensions

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