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P5381

Sigma-Aldrich

Phosphoglucose Isomerase from baker's yeast (S. cerevisiae)

Type III, ammonium sulfate suspension, ≥400 units/mg protein (biuret)

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Synonym(s):
D-Glucose-6-phosphate ketol-isomerase, PGI, Phosphosaccharomutase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

biological source

bakers yeast

Quality Level

type

Type III

form

ammonium sulfate suspension

specific activity

≥400 units/mg protein (biuret)

mol wt

tetramer 119,500 Da±600

concentration

≥3 mg/mL protein Biuret

technique(s)

activity assay: suitable

color

white

Protein ID accession no.

UniProt accession no.

storage temp.

2-8°C

Gene Information

yeast ... PGI1(852495)

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This Item
P5538P9544T2507
form

ammonium sulfate suspension

form

lyophilized powder

form

lyophilized powder

form

ammonium sulfate suspension

specific activity

≥400 units/mg protein (biuret)

specific activity

300-1,000 units/mg protein

specific activity

≥200 units/mg protein

specific activity

~10,000 units/mg protein

mol wt

tetramer 119,500 Da±600

mol wt

189 kDa

mol wt

-

mol wt

53 kDa by equilibrium centrifugation

concentration

≥3 mg/mL protein Biuret

concentration

-

concentration

-

concentration

-

technique(s)

activity assay: suitable

technique(s)

-

technique(s)

-

technique(s)

-

General description

Research area: Cell Signaling

Phosphoglucose Isomerase ubiquitous multifunctional cytosolic protein. This dimeric protein has a molecular weight of approximately 119,500 ± 600 and is composed of four subunits that are nearly identical.

Application

Phosphoglucose Isomerase from baker′s yeast (S. cerevisiae) has been used:
  • to convert fructose-6-phosphate to glucose-6-phosphate during the enzymatic measurement of serum mannose levels.
  • as one of the enzymes in the series of four enzyme-coupled reactions to measure phosphomannomutase (PMM) activity.
  • to convert fructose to glucose while evaluating fructose content in kudzu roots.

Biochem/physiol Actions

Phosphoglucose Isomerase(PGI) is an enzyme crucial for the interconversion of D-glucose 6-phosphate and D-fructose 6-phosphate. PGI is responsible for the second step of glycolysis and is involved in glucogenesis and pentose phosphate pathway (PPP). It is highly conserved in bacteria and eukaryotes. PGI plays a vital role in the survival of A. fumigatus and demonstrates diverse functions that encompass development, biosynthesis of cell wall glucan, and virulence.Phosphoglucose Isomerase functions as an isomerase, neuroleukin, autocrine motility factor, and a differentiation and maturation mediator.

Unit Definition

One unit will convert 1.0 μmole of D-fructose 6-phosphate to D-glucose 6-phosphate per min at pH 7.4 at 25 °C.

Physical form

Crystalline suspension in 2.6 M (NH4)2SO4 containing 0.1 mM magnesium acetate, pH 7.0

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Kudzu [Pueraria montana (Lour.) Merr. Variety lobata: A new source of carbohydrate for bioethanol production
Sage RF, et al.
Biomass and Bioenergy, 33, 57-61 (2009)
S L Lowe et al.
The Journal of biological chemistry, 250(1), 94-99 (1975-01-10)
Bakers' yeast phosphoglucose isomerase was studied by both chemical and physical methods to determine its submit structure. Gel filtration in 6 M guanidine HCl as well as acrylamide gel electrophoresis of sodium dodecyl sulfatedentured phosphoglucose isomerase showed two speices corresponding
Regulation of phosphoglucose isomerase/autocrine motility factor activities by the poly(ADP-ribose) polymerase family-14
Yanagawa T, et al.
Cancer Research, 67(18), 8682-8689 (2007)
Yeast Models of Phosphomannomutase 2 Deficiency, a Congenital Disorder of Glycosylation
Lao JP, et al.
G3 (Bethesda, Md.), 9(2), 413-423 (2019)
J Pozueta-Romero et al.
FEBS letters, 291(2), 233-237 (1991-10-21)
The standardized enzyme coupling method for assaying sucrose synthase activities in the direction of sucrose cleavage was reexamined using enzyme preparations from cultured cells of sycamore (Acer pseudoplatanus L.) and spinach leaves (Spinacea oleracea). Both ATP and Tris, commonly utilized

Articles

Instructions for working with enzymes supplied as ammonium sulfate suspensions

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