10128023001

NADH

Grade II, disodium salt

• 동의어(들): β-Nicotinamide adenine dinucleotide, reduced disodium salt hydrate, β-DPNH, β-NADH, DPNH, Diphosphopyridine nucleotide, reduced form, NADH
• 실험식(Hill 표기법): C₂₁H₂₇N₇Na₂O₁₄P₂ · xH₂O
• CAS Number: 606-68-8
• Molecular Weight: 709.40 (anhydrous basis)
• MDL number: MFCD03791273
• UNSPSC 코드: 12352204
• PubChem Substance ID: 329748955

속성

• Quality Level: 100
• 분석: 98%
• 형태: amorphous powder
• 분자량: (NADH-Na₂: M_r = 709.4; NADH: M_r = 665.4)
• 포장: pkg of 1 g
• 제조업체/상표: Roche
• 저장 조건: protect from light
• λmax: 260 and 340 nm
• 배송 상태: wet ice
• SMILES string: O.[Na+].[Na+].NC(=O)C1=CN(C=CC1)[C@@H]2O[C@H](COP([O-])(=O)OP([O-])(=O)OC[C@H]3O[C@H]([C@H](O)[C@@H]3O)n4cnc5c(N)ncnc45)[C@@H](O)[C@H]2O
• InChI: 1S/C21H29N7O14P2.2Na.H2O/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(32)14(30)11(41-21)6-39-44(36,37)42-43(34,35)38-5-10-13(29)15(31)20(40-10)27-3-1-2-9(4-27)18(23)33;;;/h1,3-4,7-8,10-11,13-16,20-21,29-32H,2,5-6H2,(H2,23,33)(H,34,35)(H,36,37)(H2,22,24,25);;;1H2/q;2*+1;/p-2/t10-,11-,13-,14-,15-,16-,20-,21-;;;/m1.../s1
• InChI key: FWBNCIFELNNJCX-UHOVGGJYSA-L

설명

애플리케이션

As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.

NADH has been used in MDH (malate dehydrogenase) enzymatic activity assay.

NADH has been used in lactate dehydrogenase (LDH) enzymatic activity assay, glutamate-cysteine ligase activity assay and fructose 2,6-bisphosphate assay.

생화학적/생리학적 작용

NADH is a coenzyme that functions as a regenerating electron donor in catabolic processes including glycolysis, β-oxidation and the citric acid cycle (Krebs cycle, TCA cycle). It participates in cell signaling events as well, for example as a substrate for the poly (ADP-ribose) polymerases (PARPs) during the DNA damage response. The NAD⁺/NADH dependent sirtuins play key roles in stress responses during events involving energy metabolism, with implications in cancer biology, diabetes and neurodegenerative disease.
As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.

포장

Packaged by solid weight.

품질

Purity: 98% β-NADH-Na₂ based on dry weight (enzymatically and absorbance at 340 nm), 99% (absorbance at 260 nm), A₂₆₀ nm-A₃₄₀ nm: <2.40
Organic solvents: <4% (GC)

규격

Absorbance coefficients: 14.3 x 10³ l x mol^-1 x cm^-1 at 260 nm, 6.18 x 10³ l x mol^-1 x cm^-1 at 334 nm (Hg), 6.3 x 10³ l x mol^-1 x cm^-1 at 340 nm, 3.4 x 10³ l x mol^-1 x cm^-1 at 365 nm (Hg)

Formula: C₂₁H₂₇N₇O₁₄P₂Na₂

pH value in water: 7.5 ± 0.5 (100 mg/ml)

물리적 형태

Amorphous powder

제조 메모

Activator: Mg²⁺ (5 to 10 mM)
Storage conditions (working solution): 10 mg/ml in Na-bicarbonate buffer, pH 9, contains at least 95% NADH after 4 weeks at 2 to 8 °C

저장 및 안정성

Store at 2 to 8 °C. (Store dry!)

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable