10814407001

Peroxidase (POD)

EIA grade, from horseradish

• 동의어(들): POD
• 효소 위원회 번호: 1.11.1.7 (BRENDA, IUBMB)
• UNSPSC 코드: 12352204

속성

• 생물학적 소스: horseradish
• Quality Level: 100
• 분석: 90% (HPLC)
• 형태: lyophilized (salt-free)
• 특이 활성도: ~225 U/mg lyophilizate, 25 °C (Guaiacol, H2O2)
• 포장: pkg of 100 mg
• 제조업체/상표: Roche
• 농도: 10 g/L
• 기술: activity assay: suitable
• 색상: red to brown
• 최적 pH: 6.0-6.5
• solubility: water: soluble at 68 °F
• 적합성: suitable for molecular biology
• UniProt 수납 번호: P00433
• 응용 분야: detection; life science and biopharma
• 외래 활성: ATPase <0.00100%, none detected; AcP <0.00100%; Catalase <0.700%
• 배송 상태: dry ice
• 저장 온도: −20°C (−15°C to −25°C)

설명

일반 설명

Donor: hydrogen-peroxide oxidoreductase
Peroxidase can be coupled to other proteins via its amino groups, as well as its carbohydrate moiety. The reconstituted solution can be used directly for conjugation without prior dialysis.

애플리케이션

Peroxidase (POD) has been used in the spermine oxidase enzyme activity assay.

서열

Blocking substances
Preparations does not contain substances that block the amino groups, but in contrast to other enzymes POD has only 2 to 3 free amino groups which are not accessible; even by extended dialysis of POD, the number of NH₂-groups/mol does not change.

POD
POD contains 1 molecule of protoheme IX as prosthetic group and 2 molecules of Ca²⁺.
POD exists in > 10 isoenzymic forms (some sources estimate approx. 40 isoenzymes).

Carbohydrate Content
12 to 14.5 mg/100 mg Iyophilizate

Free Amino Groups
Free amino groups: 2 to 3 mol/mol enzyme

제조 메모

Activator: Ammonia, pyridine, imidazole, all at pH >7; detergents
Working concentration: A suggested working ratio, for immediate use in coupling procedures, is 2 mg POD to 4 mg lgG (molar ratio = 2:1, POD:IgG).
Specific activity: approximately 225U/mg lyophilizate (+25°C, guaiacol, H₂O₂) approximately 1,000 U/mg lyophilizate (+25°C, ABTS, H₂O₂, pH 5.0) approximately 3,500 U/mg lyophilizate (+25°C, tetramethylbenzidine, H₂O₂)
Amino groups: 2 - 3moles/mole enzyme

Carbohydrates: 12.0 - 14.5% (w/w)
Isoenzymes: ≥90% isoenzyme C (HPLC)
Purity: single peak (HPLC)
Purity number: 3.0 - 3.5 (A₄₀₃/A₂₇₅)

저장 및 안정성

Keep container tightly closed in a dry and well-ventilated place.

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H317,H334
• 예방조치 성명서: P261 - P280 - P284 - P304 + P340 - P333 + P313 - P342 + P311
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable