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Merck
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주요 문서

11051482001

Roche

dTTP

Lithium salt

동의어(들):

Thymidine 5′-triphosphate sodium salt, 2′-deoxy-thymidine-5′-triphosphate, deoxy-thymidine-5′-triphosphate, 2′-, TTP, dTTP

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About This Item

실험식(Hill 표기법):
C10H16N2O14P3Na
CAS Number:
Molecular Weight:
504.15
MDL number:
UNSPSC 코드:
41116100
PubChem Substance ID:

Quality Level

분석

≥98%

형태

solution

사용

sufficient for 6,250 standard reactions (PCR)

포장

pkg of 250 μL (25 μmol)

제조업체/상표

Roche

농도

100 mM

기술

DNA amplification: suitable
DNA sequencing: suitable

저장 온도

−20°C

InChI

1S/C10H17N2O14P3.Na.H/c1-5-3-12(10(15)11-9(5)14)8-2-6(13)7(24-8)4-23-28(19,20)26-29(21,22)25-27(16,17)18;;/h3,6-8,13H,2,4H2,1H3,(H,19,20)(H,21,22)(H,11,14,15)(H2,16,17,18);;

InChI key

KAELIXTVSANDSE-UHFFFAOYSA-N

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일반 설명

dTTP is a 100 mM clear, colorless solution of lithium salt (pH 7).
dTTP solution in water (pH 7), 100 mM, lithium salt.

애플리케이션

Use dTTP, lithium salt, for DNA sequencing, labeling, and all types of amplification reactions or primer-extension reactions.
It has been used in PCR fingrprinting and PCR techniques.

품질

Typical analysis:
  • ≥98% dTTP (HPLC), ≤1.5% dTDP (HPLC), ≤0.5% dTMP (HPLC)
  • Absorbance determination A250/A260, A280/A260, A290/A260
  • DNase activity negative, according to the current Quality Control procedures
  • Suitability for PCR

단위 정의

1 μmol dTTP = 9.5 A260units

기타 정보

For life science research only. Not for use in diagnostic procedures.

Our PCR Grade deoxynucleotides are even purer than the lithium salts, to ensure excellent performance in your PCRs. PCR Grade deoxynucleotides contain the sodium salt of the nucleotide in PCR Grade Water.
For your convenience, some of our deoxynucleotides are also available in:
  • nucleotide sets (all 4 nucleotides in separate vials)
  • ready-to-use nucleotide mixes (premixed solutions of all 4 nucleotides)

They are also vital components of:
  • kits (dNTPs, buffer, and polymerase in separate vials)
  • ready-to-use mixes [polymerase, nucleotides, and buffer (premixed 2x solutions)]

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point (°F)

No data available

Flash Point (°C)

No data available


시험 성적서(COA)

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문서 라이브러리 방문

Luciana Trilles et al.
Memorias do Instituto Oswaldo Cruz, 103(5), 455-462 (2008-09-18)
The molecular types of 443 Brazilian isolates of Cryptococcus neoformans and Cryptococcus gattii were analyzed to determine their geographic distribution within Brazil and their underlying host conditions. The following data, imported from previous epidemiological studies as well as two culture
Wieland Meyer et al.
Emerging infectious diseases, 9(2), 189-195 (2003-02-27)
A network was established to acquire basic knowledge of Cryptococcus neoformans in IberoAmerican countries. To this effect, 340 clinical, veterinary, and environmental isolates from Argentina, Brazil, Chile, Colombia, Mexico, Peru, Venezuela, Guatemala, and Spain were typed by using M13 polymerase
A Pillay et al.
Journal of clinical microbiology, 42(8), 3853-3856 (2004-08-07)
An immunochromatographic strip test, Xenostrip-Tv, was compared to wet mount and PCR for the diagnosis of Trichomonas vaginalis infection in women. Of 428 specimens tested, 54 (12.6%) were positive by an "expanded gold standard," defined as either a positive wet

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