DGTP-RO

dGTP

PCR Grade, sodium salt

• 동의어(들): 2′-Deoxyguanosine 5′-triphosphate trisodium salt solution, dGTP-Na₃
• 실험식(Hill 표기법): C₁₀H₁₃N₅Na₃O₁₃P₃
• CAS Number: 93919-41-6
• Molecular Weight: 573.13
• MDL number: MFCD00080337
• UNSPSC 코드: 12352302
• PubChem Substance ID: 329798919

속성

• Quality Level: 100
• 분석: 99% (dNTP)
• 형태: solution
• 사용: sufficient for 125,000 standard reactions (03732703001); sufficient for 31,250 standard reactions (11969030001); sufficient for 6,250 standard reactions (11934538001)
• 포장: pkg of 1,250 μL (11969030001 [100 mM]); pkg of 4 × 1,240 μL (03732703001 [4x100 mM]); pkg of 250 μL (11934538001 [100 mM])
• 제조업체/상표: Roche
• 농도: <0.9% (dNDP); 100 mM
• 저장 온도: −20°C
• SMILES string: [Na+].[Na+].[Na+].NC1=Nc2c(ncn2[C@H]3C[C@H](O)[C@@H](COP([O-])(=O)OP([O-])(=O)OP(O)([O-])=O)O3)C(=O)N1
• InChI: 1S/C10H16N5O13P3.3Na/c11-10-13-8-7(9(17)14-10)12-3-15(8)6-1-4(16)5(26-6)2-25-30(21,22)28-31(23,24)27-29(18,19)20;;;/h3-6,16H,1-2H2,(H,21,22)(H,23,24)(H2,18,19,20)(H3,11,13,14,17);;;/q;3*+1/p-3/t4-,5+,6+;;;/m0.../s1
• InChI key: IWGGLKOTEOCWQP-BIHLCPNHSA-K

설명

일반 설명

2′-Deoxyguanosine 5′-triphosphate (dGTP) is made of a guanine nucleobase attached to deoxyribose and a 5′-hydroxyl on the sugar linked to a chain of three phosphate residues. It is used by the cells for the synthesis of DNA with the help of DNA polymerase.

dGTP, PCR Grade, is a 100 mM clear, colorless solution of the sodium salt (pH 8.3). PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).

애플리케이션

2′-Deoxyguanosine 5′-triphosphate trisodium salt is utilized for methods such as DNA sequencing, PCR and other DNA polymerase based DNA synthesis and repair techniques.

품질

Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.

물리적 형태

solution of dGTP in water (pH 8.3).

제조 메모

Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade).

기타 정보

For general laboratory use.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash