추천 제품
생물학적 소스
sheep
Quality Level
결합
alkaline phosphatase conjugate
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
polyclonal
형태
solution
포장
pkg of 200 μL (150 U)
제조업체/상표
Roche
동형
IgG
저장 온도
2-8°C
일반 설명
Digoxigenin is a hapten, useful in labeling and detection of nucleic acids. This product contains Fab fragments from polyclonal anti-digoxigenin antibodies, conjugated to alkaline phosphatase. Anti-Digoxigenin-AP, Fab fragments are useful for the detection of digoxigenin-labeled compounds.
특이성
The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.
Heat inactivation: yes
Heat inactivation: yes
애플리케이션
Use Anti-Digoxigenin-AP, Fab fragments for the detection of digoxigenin-labeled compounds using:
- cDNA array
- Colony/plaque hybridization
- Dot blot
- ELISA
- Gel shift assay
- Immunohistocytochemistry
- In situ hybridization
- Nonradioactive DNA sequencing blot
- Northern blot
- RNase protection assay
- Southern blot
- Western blot
- Fluorescent in situ hybridization
- Section in situ hybridization and whole mount in situ hybridization
- Electrophoretic mobility shift assay
제조 메모
After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated to the specific label, and stabilized in buffer.
Working concentration: Working concentration of conjugate will depend on the application and substrate. The following concentrations should be taken as a guideline:
Working solution: Northern blot, Southern blot
100 mM Maleic acid, 150 mM NaCl, pH 7.5.
Western blot
50 mM Tris-HCl, 150 mM NaCl, pH 7.5
other applications
100 mM Tris-HCl, 150 mM NaCl, pH 7.5
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.
Storage conditions (working solution): Diluted conjugate is stable at 2 to 8 °C for 12 hours.
Always prepare freshly!
Working concentration: Working concentration of conjugate will depend on the application and substrate. The following concentrations should be taken as a guideline:
- Dot blot: 150 mU/ml
- ELISA: 150 to 300 mU/ml
- Immunohistocytochemistry: 250 to 500 mU/ml
- In situ hybridization: 1.5 to 7.5 U/ml
- Southern blot: 150 mU/ml
- Western blot: 250 to 500 mU/ml
Working solution: Northern blot, Southern blot
100 mM Maleic acid, 150 mM NaCl, pH 7.5.
Western blot
50 mM Tris-HCl, 150 mM NaCl, pH 7.5
other applications
100 mM Tris-HCl, 150 mM NaCl, pH 7.5
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.
Storage conditions (working solution): Diluted conjugate is stable at 2 to 8 °C for 12 hours.
Always prepare freshly!
분석 메모
- Cross reactivity to digitoxin and digitoxigenin: <1 %
- No cross reactivity with other human estrogen or androgen steroids, e.g. estradiol or testosterone
- Cross reactivity with digoxin: not known
- Conjugate does not bind to itself at all
- Normally one molecule of the conjugate binds to one molecule digoxigenin, although ther are two possible binding sites for digoxigenin
- Nonspecific binding to RNA is not expected
기타 정보
For life science research only. Not for use in diagnostic procedures.
적합한 제품을 찾을 수 없으신가요?
당사의 제품 선택기 도구.을(를) 시도해 보세요.
WGK
WGK 1
Flash Point (°F)
No data available
Flash Point (°C)
No data available
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
![CDP-Star® Disodium 2-chloro-5-(4-methoxyspiro {1,2-dioxetane-3,2′-(5′-chloro)tricyclo[3.3.1.13,7 ]decan}-4-yl)-1-phenyl phosphate](/deepweb/assets/sigmaaldrich/product/structures/224/846/d08963f5-c8ba-42e6-b4f7-9ee7fd76d809/640/d08963f5-c8ba-42e6-b4f7-9ee7fd76d809.png)
Exorhodopsin and melanopsin systems in the pineal complex and brain at early developmental stages of Atlantic halibut (Hippoglossus hippoglossus).
Eilertsen M, et al.
The Journal of Comparative Neurology, 522(18), 4003-4022 (2014)
Use of In Situ Hybridization to Examine Gene Expression in the Embryonic, Neonatal, and Adult Urogenital System.
Rumballe B A, et al.
Kidney Development: Methods and Protocols, 223-239 (2012)
The digoxigenin (DIG) system for non-radioactive labelling and detection of nucleic acids-an overview.
<BIG>Holtke HJ, et al.</BIG>
Cellular and molecular biology (Noisy-le-Grand, France), 41, 883-905 (1995)
Jer-Cherng Chang et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(32), 13371-13376 (2009-08-12)
A signature of eclosion hormone (EH) action in insect ecdysis is elevation of cGMP in Inka cells, leading to massive release of ecdysis triggering hormone (ETH) and ecdysis initiation. Although this aspect of EH-induced signal transduction is well known, the
Tara C Carlisle et al.
The Journal of comparative neurology, 522(4), 861-875 (2013-08-14)
Electrical synapses are expressed prominently in the developing and mature nervous systems. Unlike chemical synapses, little is known about the developmental role of electrical synapses, reflecting the limitations imposed by the lack of selective pharmacological blockers. At a molecular level
문서
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.