추천 제품
양식
solution
Quality Level
포장
pkg of 100 mL
제조업체/상표
Roche
색상
dark purple
solubility
H2O: insoluble
배송 상태
wet ice
저장 온도
2-8°C
일반 설명
BM purple is a chromogenic substrate for alkaline phosphatase (AP) designed for precipitating enzyme immunoassays. It develops a permanent, dark purple band or spot at the AP binding site on the membrane or solid support. The stabilized substrate solution may be used directly from the bottle with no mixing or reconstitution. It gives less background staining than when using the BCIP/NBT two-bottle system, without a loss of sensitivity. The reaction product has a dark purple color and is insoluble in water. The dark purple color can be visually seen.
Appearance prior to use: light yellow color;
After color development: dark purple
Appearance prior to use: light yellow color;
After color development: dark purple
애플리케이션
- Colorimetric AP substrate for: Immunohistocytochemistry
- Western blot
- Southern blot
- Northern blot
- Colony and plaque hybridization
- In situ hybridization
성분
Ready-to-use solution.
제조 메모
Working concentration: Best results have been obtained using 200 mU POD and casein as blocking reagent.
Working solution: Solubility in water: insoluble.
Working solution: Solubility in water: insoluble.
기타 정보
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
does not flash
Flash Point (°C)
does not flash
이미 열람한 고객
Christoph Kessle
Nonradioactive Analysis of Biomolecules null
Valentina Garcia-Lee et al.
The International journal of developmental biology, 65(4-5-6), 395-401 (2020-09-16)
The cell differentiation of the musculoskeletal system is highly coordinated during limb development. In the distal-most region of the limb, WNT and FGF released from the apical ectodermal ridge maintain mesenchymal cells in the undifferentiated stage. Once the cells stop
Jonas Malkmus et al.
Nature communications, 12(1), 5557-5557 (2021-09-23)
Precise cis-regulatory control of gene expression is essential for normal embryogenesis and tissue development. The BMP antagonist Gremlin1 (Grem1) is a key node in the signalling system that coordinately controls limb bud development. Here, we use mouse reverse genetics to
Ho Chi Leung et al.
Zygote (Cambridge, England), 1-12 (2021-09-18)
It has previously been reported that in ex vivo planar explants prepared from Xenopus laevis embryos, the intracellular pH (pHi) increases in cells of the dorsal ectoderm from stage 10.5 to 11.5 (i.e. 11-12.5 hpf). It was proposed that such
Chunyan Li et al.
Nature communications, 12(1), 1094-1094 (2021-02-19)
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