추천 제품
분석
97.7% (HPLC)
Quality Level
양식
solution
분자량
Mr 987.5 (biotin-16-UTP-Li4)
포장
pkg of 25 μL (250 nmol; 10mM)
제조업체/상표
Roche
색상
colorless
solubility
water: miscible
배송 상태
dry ice
저장 온도
−20°C
일반 설명
For biotin-16-UTP, biotin is bound to uridine triphosphate via an amide linkage. Biotin-16-UTP is a substrate for SP6, T3, and T7 RNA polymerase. It can replace UTP in the in vitro transcription reaction for RNA labeling. Linearized template DNA with T7, SP6, or T3 promoter is in vitro-transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and Biotin-16-UTP, respectively.
애플리케이션
Biotin-labeled RNA can be used as a hybridization probe for:
- Northern blots
- Southern blots
- Plaque or colony lifts
- RNase protection experiments
- In situ hybridization
- Microarray hybridization
품질
Typical analysis: At least 85% Biotin-16-UTP (HPLC, area%).
물리적 형태
Biotin-16-UTP, tetralithium salt, 10mM solution
기타 정보
For life science research only. Not for use in diagnostic procedures.
WGK
WGK 1
Flash Point (°F)
does not flash
Flash Point (°C)
does not flash
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