11427857910

Fluorescein-12-UTP

≥85% (HPLC), solution

• 동의어(들): Fluorescein-12-UTP tetralithium salt, Fluorescein-5(6)-carboxamidocaproyl-(5-[3-aminoallyl]uridine 5′-triphosphate)
• 실험식(Hill 표기법): C₃₉H₄₁N₄O₂₂P₃
• CAS Number: 134367-01-4
• Molecular Weight: 1010.68
• MDL number: MFCD00866062
• UNSPSC 코드: 41116100

속성

• Quality Level: 100
• 분석: ≥85% (HPLC)
• 양식: solution
• 분자량: 1034.4
• 포장: pkg of 25 μL (250 nmol; 10 mM)
• 제조업체/상표: Roche
• 저장 온도: −20°C

설명

일반 설명

Quality
Typical analysis: At least 85% Fluorescein-12-UTP (HPLC, area%).

Fluorescein is bound to uridine triphosphate via an amide linkage. Molecular formula is C₃₉H₃₇N₄O₂₂P₃Li₄.

애플리케이션

Fluorescein-12-UTP is used as a substrate for SP6, T3, and T7 RNA polymerases. It can replace UTP in the in vitro transcription reaction for RNA labeling.
Labeled RNA can be subsequently detected by in situ hybridization and direct fluorescence detection or detection by ELISA using Anti-Fluorescein-AP, Fab fragments or Anti-Fluorescein-POD, Fab fragments.

기타 정보

For life science research only. Not for use in diagnostic procedures.

For your convenience, fluorescein-UTP is also available in a convenient labeling mix (single solution with conventional NTPs and Fluorescein-12-UTP).

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash