11699105001

PCR Buffer Without MgCl₂, 10x concentrated

solution, pkg of 3 × 1 mL

• 동의어(들): buffer
• UNSPSC 코드: 41105600

속성

• 형태: solution
• Quality Level: 100
• 포장: pkg of 3 × 1 mL
• 제조업체/상표: Roche
• 저장 온도: −20°C

설명

일반 설명

The PCR buffer without MgCl₂, in combination with the MgCl₂ stock solution, is used for the individual adjustment of the Mg²⁺ concentration in the PCR reaction. In most applications a concentration of 1.5mM MgCl₂ will yield satisfactory results with a dNTP concentration of 200μM each. In many cases, however, it is required to titrate the optimal Mg²⁺ concentration to increase the specificity and yield of the PCR reaction.The buffer is ten-fold concentrated and should be used in combination with the MgCl₂ stock solution.

애플리케이션

The PCR buffer without MgCl₂ has been used for the amplification of specific DNA fragments by the polymerase chain reaction (PCR).

물리적 형태

Solution, 100 mM Tris-HCl, 500 mM KCl (pH 8.3 at 20 °C)

기타 정보

For general laboratory use.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash