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Merck
모든 사진(2)

주요 문서

M1028

Sigma-Aldrich

Magnesium chloride solution

for molecular biology, 1.00 M±0.01 M

동의어(들):

MgCl2, dichloromagnesium, magnesium chloride

로그인조직 및 계약 가격 보기


About This Item

Linear Formula:
MgCl2
CAS Number:
Molecular Weight:
95.21
MDL number:
UNSPSC 코드:
12352302
PubChem Substance ID:
NACRES:
NA.52
solubility:
water: soluble

Grade

for molecular biology

Quality Level

무균

non-sterile; 0.2 μm filtered

양식

liquid

농도

1.00 M±0.01 M

기술

DNA sequencing: suitable

불순물

DNAse, none detected
NICKase, none detected
Protease, none detected
RNAse, none detected

solubility

water: soluble

density

1.080 g/cm3

양이온 미량물

heavy metals (as Pb): ≤5 ppm

적합성

suitable for electrophoresis
suitable for molecular biology

응용 분야

cell analysis

외래 활성

DNase, RNase, NICKase and protease, none detected

SMILES string

Cl[Mg]Cl

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

InChI key

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

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관련 카테고리

일반 설명

Magnesium chloride is a common ionic halide that readily dissolves in water. It plays various crucial biological roles in enzymology, cell membrane/wall structure, muscle cell physiology, and nucleic acid composition. Additionally, magnesium chloride serves as an essential co-factor in numerous enzymes, including DNAse, specific restriction enzymes, and Ribonuclease H. It is widely utilized to provide magnesium ions in diverse molecular biology applications, including PCR reactions.

애플리케이션

Magnesium chloride is widely used to supply the magnesium ion in various molecular biology applications, including PCR reactions and buffers. It has been used -
  • to prepare buffer to lyse cells for immunoprecipitation
  • as a supplement in Krebs-Henseleit buffer in chronic high-fat diet studies of aged mice.
  • in embedding 3-dimensional mtgase hydrogels in invitro studies of osteocyte differentitaion and interconnected cellular networks.
  • It has been used to protect the liver from oxidative damage.
  • to prepare serum-free medium for electrophysiological studies of rat brain slice cultures.

특징 및 장점

  • Available as a Ready-to-use solution which reduces the need for preparation time.
  • Heavy metals (as Pb): ≤5 ppm
  • Free from DNase, RNase, NICKase and protease

성분

Magnesium chloride solution contains 1M magnesium chloride in 18 megaohm water.

기타 정보

For additional information on our range of Biochemicals, please complete this form.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves


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문서 라이브러리 방문

Protective Effects of Magnesium Chloride on Liver Enzymes and Biomarkers of Oxidative Stress in high fat diet fed Rats
Mohammed et, al
Nigerian Journal of Physiological Sciences : Official Publication of the Physiological Society of Nigeria, 34(2), 149-157 (2019)
Yelena Y Grinberg et al.
Journal of neurochemistry, 122(1), 221-229 (2012-04-25)
Spreading depression (SD), the likely cause of migraine aura and perhaps migraine, is triggered by widespread and unfettered neuronal hyperexcitability. Migraine and the initiating hyperexcitability of seizure, which involve oxidative stress (OS), are likely interrelated. Environmental enrichment (EE) decreases seizure
M J Mc Garrigle et al.
European cells & materials, 31, 323-340 (2016-05-25)
Extracellular matrix (ECM) stiffness and cell density can regulate osteoblast differentiation in two dimensional environments. However, it is not yet known how osteoblast-osteocyte differentiation is regulated within a 3D ECM environment, akin to that existing in vivo. In this study
Antimo Migliaccio et al.
Methods in molecular biology (Clifton, N.J.), 776, 361-370 (2011-07-29)
Much evidence indicates that, with few exceptions, non-genomic actions of steroids are mediated by receptors universally known as nuclear receptors. Steroid receptors do not exhibit intrinsic tyrosine kinase activity. Nevertheless, they stimulate different signaling pathways in cytoplasm of target cells
Hiroaki Eshima et al.
Journal of applied physiology (Bethesda, Md. : 1985), 128(5), 1153-1162 (2020-03-28)
Obesity and aging reduce skeletal muscle contractile function. However, it remains unclear whether obesity additively promotes muscle contractile dysfunction in the setting of aging. In this study, we investigated skeletal muscle contractile function ex vivo and intracellular Ca2+ release in

프로토콜

This procedure may be used for Mutanolysin products.

Measure hexokinase activity using a continuous spectrophotometric rate-determination assay at 340 nm, catalyzing D-hexose sugar phosphorylation using ATP.

Enzymatic assay of lipase type XIII from Pseudomonas sp. using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 3.1.1.3)

Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

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