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Merck
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주요 문서

AGRLE-RO

Roche

Agarose LE

low electroendosmosis

동의어(들):

agarose

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About This Item

UNSPSC 코드:
12352200

생물학적 소스

algae (seaweed)

양식

powder

포장

pkg of 100 g (11685660001)
pkg of 500 g (11685678001)

제조업체/상표

Roche

EEO

0.05 -0.13

전이 온도

gel point 36 °C ±1.5

겔 강도

>=2,500 g/cm2

외래 활성

Dnase, none detected
Rnase, none detected

배송 상태

ambient

저장 온도

20-25°C

일반 설명

Agarose is obtained from seaweed and contains repeated agarobiose units. During agarose gel formation, the polymers aggregate to form a network with varying pore sizes. This property is used in agarose gel electrophoresis to separate DNA.

애플리케이션

Agarose LE has been used for:

  • analyzing fragments between 0.2 and 15 kb
  • analysis of PCR products
  • examination of restriction endonucleases
  • digests of plasmid, cosmid, and λ phage DNA
  • electrophoresis of RNA in, e.g., denaturing gels containing formaldehyde
  • in combination with ethidium bromide ​in DNA electrophoresis to facilitate the separation and visualization of DNA fragments.
Nucleic acid fragments separated with Agarose LE can be blotted to nylon or nitro-cellulose membranes by all standard blotting techniques.
Important Note: Detection with nonradioactive probes e.g., digoxigenin-labeled nucleic acids, does not interfere with the use of Agarose LE.

품질

Absence of DNase: none detected according to the current Quality Control procedures.
Absence of RNase: none detected according to the current Quality Control procedures.

기타 정보

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

does not flash

Flash Point (°C)

does not flash


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이미 열람한 고객

Slide 1 of 1

1 of 1

"Impact of Interleukin 16 (IL-16) Gene Polymorphism among

Seropositive Stages in HIV-1 Infected Patients in North India.
Kakkar K
Journal of Virology & Antiviral Research, 8, 006-011 (2016)
Pei Yun Lee et al.
Journal of visualized experiments : JoVE, 62(62), 3923-3923 (2012-05-02)
Agarose gel electrophoresis is the most effective way of separating DNA fragments of varying sizes ranging from 100 bp to 25 kb(1). Agarose is isolated from the seaweed genera Gelidium and Gracilaria, and consists of repeated agarobiose (L- and D-galactose)
Emily Hodges et al.
Nature protocols, 4(6), 960-974 (2009-05-30)
Complementary techniques that deepen information content and minimize reagent costs are required to realize the full potential of massively parallel sequencing. Here, we describe a resequencing approach that directs focus to genomic regions of high interest by combining hybridization-based purification

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