PRON-RO

Pronase

from Streptomyces griseus

• 동의어(들): non-specific protease
• 효소 위원회 번호: 3.4.24.4 (BRENDA, IUBMB)
• UNSPSC 코드: 12352204

속성

• 생물학적 소스: Streptomyces griseus
• Quality Level: 100
• 무균: non-sterile
• 형태: lyophilized powder
• 포장: pkg of 1 g (10165921001); pkg of 5 g (11459643001)
• 제조업체/상표: Roche
• 농도: 0.5-2 mg/mL
• 파라미터: 35-40 °C optimum reaction temp.
• 기술: DNA extraction: suitable
• 색상: white
• 최적 pH: 7.0-8.0
• pH 범위: 6.0-9.0
• solubility: water: soluble 10-20 g/L
• 적합성: suitable for molecular biology
• NCBI 수납 번호: WP_012381357.1
• UniProt 수납 번호: B1W0S5; P00776
• 응용 분야: life science and biopharma
• 외래 활성: Deoxyribonuclease <0.002 U/mg ( based on lyoph.); Ribonuclease <0.002 U/mg ( based on lyoph.)
• 저장 온도: 2-8°C
• 유전자 정보: Streptomyces griseus ... SGR_RS27460(6214424)

설명

일반 설명

at 40°C with casein as the substrate, pH 7.5, equivalent to approximately 1270 PU/mg or approximately 25 PUK/mg.

특이성

Pronase has a broad specificity, breaking down virtually all proteins into their individual amino acids; resolves carboxylic acids and alcohols.

애플리케이션

Use Pronase to completely hydrolyze proteins in research applications. Pronase is used for the degradation of proteins during the isolation of DNA and RNA, such as in the extraction of phage DNA or the isolation of plasmid DNA. It is not necessary to let pronase self-digest prior to use. It is also used in histochemistry and cell culture for tissue dissociation in conjunction with collagenase and trypsin, and for the production of glycopeptides from purified glycoproteins. It has been used for the dechorionation of Zebrafish embryos.

생화학적/생리학적 작용

Pronase has wide-ranging specificity. Therefore, it is commonly used when extensive or complete degradation of proteins is required. Its applications include protein analysis in cell organelles, removal of proteins during DNA and RNA isolation, and the production of protein hydrolysates for amino acid analysis. In addition, Pronase surpasses trypsin in its ability to disperse fibroblastic cell lines, excelling in both monolayer detachment and the creation of single-cell suspensions.

단위 정의

A unit of non-specific protease activity increases the rate of release of folin-positive amino acids and peptides from casein, at +40 °C and pH 7.5. For one unit (U) 1 μmol/min tyrosine is released, and for the unit "PU", the value is 1 μg/minute (1 U = 181 PU); for the unit "PUK", it is 0.1/minute (measured is the change in absorbance of molybdenum blue, formed by reaction with the folin ′s reagent under conditions such that 1 PUK = 50 PU).

제조 메모

Stabilizers: Protective effect of calcium ions

The preparation contains 20% calcium acetate for stability. It is free of starch as per the current Quality Control Procedures.The activity of a diluted solution containing 0.01–0.1 M calcium was stable over 24 hours at neutral pH at 4 to 8 °C. Pronase is also protected from heat inactivation by low levels of calcium.
Working concentration: 0.5 to 2 mg/ml
Working solution: Solvent is recommended in distilled water.
Stock solution is prepared by adding pronase powder to distilled water (10 to 20 mg/ml).
Storage conditions (working solution): -15 to -25 °C

재구성

The lyophilizate is soluble in water (10 to 20 mg/ml).

저장 및 안정성

Store dry

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• 픽토그램: GHS07,GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H315 - H319 - H334 - H335
• 예방조치 성명서: P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash