16669

Magenta-Gal

reagent for selection of recombinant bacterial clones

• 동의어(들): 5-Bromo-6-chloro-3-indolyl-β-D-galactopyranoside, Mag-Gal
• 실험식(Hill 표기법): C₁₄H₁₅BrClNO₆
• CAS Number: 93863-88-8
• Molecular Weight: 408.63
• Beilstein: 1552602
• MDL number: MFCD00210022
• UNSPSC 코드: 12352200
• PubChem Substance ID: 329751317

속성

• Grade: for molecular biology
• Quality Level: 100
• 무균: non-sterile
• 분석: ≥98.0% (HPLC)
• 형태: powder
• solubility: DMF: soluble; DMSO: soluble
• 적합성: suitable for β-galactosidase test
• 저장 온도: −20°C
• SMILES string: OC[C@H]1O[C@@H](Oc2c[nH]c3cc(Cl)c(Br)cc23)[C@H](O)[C@@H](O)[C@@H]1O
• InChI: 1S/C14H15BrClNO6/c15-6-1-5-8(2-7(6)16)17-3-9(5)22-14-13(21)12(20)11(19)10(4-18)23-14/h1-3,10-14,17-21H,4H2/t10-,11-,12+,13-,14-/m1/s1
• InChI key: CHRVKCMQIZYLNM-RKQHYHRCSA-N

설명

일반 설명

Magenta-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Magenta-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

애플리케이션

Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. Also suitable for use immunoblotting and immunocytochemical assays.

원리

When Magenta-Gal is hydrolyzed by β-galactosidase, the resulting product will form a red precipitate. Lac+ colonies grown in the presence of Magenta-Gal turn an intense red color, allowing for easy differentiation between lac+ and lac- colonies.

안전성 정보

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)