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Merck
모든 사진(3)

주요 문서

B2904

Sigma-Aldrich

Bluo-Gal

reagent for selection of recombinant bacterial clones

동의어(들):

5-Bromo-3-indolyl β-D-galactopyranoside, substrate for β-galactosidase, Bluo-Gal

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About This Item

실험식(Hill 표기법):
C14H16BrNO6
CAS Number:
Molecular Weight:
374.18
Beilstein:
1550140
MDL number:
UNSPSC 코드:
12352200
PubChem Substance ID:
NACRES:
NA.85

Grade

for molecular biology

Quality Level

무균

non-sterile

분석

≥98% (HPLC)

형태

powder

기술

nucleic acid detection: suitable

solubility

DMF: soluble

적합성

suitable for substrate for β-galactosidase

저장 온도

−20°C

SMILES string

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H16BrNO6/c15-6-1-2-8-7(3-6)9(4-16-8)21-14-13(20)12(19)11(18)10(5-17)22-14/h1-4,10-14,16-20H,5H2/t10-,11+,12+,13-,14-/m1/s1

InChI key

LINMATFDVHBYOS-MBJXGIAVSA-N

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일반 설명

Bluo-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Bluo-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

애플리케이션

Bluo-Gal is suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. Bluo-Gal is suitable for use as β-galactosidase substrate in the staining of skin tissues for electron microscopy analysis, Dictyostelium samples and in the staining of vena cava of adult mice.
Chromogenic substrate suitable for identification of lac+ bacterial colonies. An alternative to 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal), producing a darker blue color.

원리

When Bluo-Gal is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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