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Merck
모든 사진(2)

주요 문서

199664

Sigma-Aldrich

Sudan Black B

certified by the Biological Stain Commission

동의어(들):

Ceres black BN, Fat Black HB, Solvent Black 3

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About This Item

실험식(Hill 표기법):
C29H24N6
CAS Number:
Molecular Weight:
456.54
색상 지수 번호:
26150
Beilstein:
723248
EC Number:
MDL number:
UNSPSC 코드:
12171500
PubChem Substance ID:
NACRES:
NA.47

Grade

certified by the Biological Stain Commission

Quality Level

양식

powder

기술

microbe id | staining: suitable

색상

dark brown to very dark brown

mp

120-124 °C (lit.)

λmax

415 nm (2nd)
598 nm

ε (흡광계수)

≥20000 at 596-603 nm in ethanol

응용 분야

diagnostic assay manufacturing
hematology
histology

저장 온도

room temp

SMILES string

CC1(C)Nc2cccc3c(ccc(N1)c23)\N=N\c4ccc(\N=N\c5ccccc5)c6ccccc46

InChI

1S/C29H24N6/c1-29(2)30-26-14-8-13-22-25(17-18-27(31-29)28(22)26)35-34-24-16-15-23(20-11-6-7-12-21(20)24)33-32-19-9-4-3-5-10-19/h3-18,30-31H,1-2H3/b33-32+,35-34+

InChI key

YCUVUDODLRLVIC-VPHDGDOJSA-N

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애플리케이션

Sudan Black B has been used in brain BLAQ (Block Lipids and Aldehyde Quench) technique and is used in lipid detection.

생화학적/생리학적 작용

Sudan Black B has been used to stain phospholipids and intracellular lipids. Staining pattern is almost similar to myeloperoxidase reaction, with positive staining of granulocytic cells, eosinophils and weak monocytic staining.It doesn′t stain lymphocytes. Sudan Black B can be used to stain older blood or bone marrow smears, but the stain slightly fades over time. It is considered to be a most sensitive and specific reagent for lipid staining. Sudan Black B stains phospholipids gray and neutral fats blue-black.

적합성

Certified as a fat staining using the Churukian variant of the Lillie-Ashburn supersaturated isopropanol method. Sudan III is substituted for the oil red O in the procedure. Burdon′s stain for bacterial lipid.

픽토그램

Health hazard

신호어

Warning

유해 및 위험 성명서

Hazard Classifications

Muta. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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Lot/Batch Number

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문서 라이브러리 방문

이미 열람한 고객

H N Subramaniam et al.
Journal of biochemical and biophysical methods, 21(1), 9-16 (1990-06-01)
The selective staining of neutral lipids in Human Amnion cells in tissue culture was achieved using a particular fraction of the lipid dye, Sudan black B and a standardized staining procedure. The fraction, termed SBB-I, was isolated by thin-layer chromatography.
Effects of feeding different lipid sources on hepatic histopathology features and growth traits of broiler chickens
Tufarelli V, et.al.
Acta Histochemica, 117, 780-783 (2015)
Acetylated Sudan Black B as a More Specific Histochemical Reagent for Lipides
CASSELMAN WGB
Journal of Cell Science, 321- 322 (1954)
John P. Greer
Wintrobe's Clinical Hematology, 1 (2009)
Brain BLAQ: Post-hoc thick-section histochemistry for localizing optogenetic constructs in neurons and their distal terminals
Kupferschmidt DA
Frontiers in Neuroanatomy (2015)

문서

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