76307

Pefabloc^® SC

analytical standard

• 동의어(들): 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, AEBSF
• 실험식(Hill 표기법): C₈H₁₀FNO₂S · HCl
• CAS Number: 30827-99-7
• Molecular Weight: 239.69
• Beilstein: 7604627
• MDL number: MFCD00132962
• UNSPSC 코드: 41116107
• PubChem Substance ID: 329767025
• NACRES: NA.24

속성

• Grade: analytical standard
• Quality Level: 200
• 분석: ≥95.0% (HPLC)
• 형태: powder or crystals
• 유통기한: 5 yr
• 기술: HPLC: suitable; gas chromatography (GC): suitable
• 응용 분야: forensics and toxicology; pharmaceutical (small molecule)
• 형식: neat
• 저장 온도: 2-8°C
• SMILES string: FS(C1=CC=C(CCN)C=C1)(=O)=O.[H]Cl
• InChI: 1S/C8H10FNO2S.ClH/c9-13(11,12)8-3-1-7(2-4-8)5-6-10;/h1-4H,5-6,10H2;1H
• InChI key: WRDABNWSWOHGMS-UHFFFAOYSA-N

설명

일반 설명

Pefabloc^® SC is a potent, nontoxic and relatively easy to use, irreversible serine proteinase inhibitor. It is reported to efficiently inhibit the platelet activating factor (PAF)-degrading acetylhydrolase (acetylhydrolase) in both humans and rats.

Pefabloc^® SC is a trypsin inhibitor, which can be used in the synthesis of its arginine-like analogue, 4-(2-guanidinoethyl)benzenesulfonyl fluoride.

애플리케이션

Pefabloc^® SC may be used as an analytical standard in determining the native oligomeric state and substrate specificity of rat NTPDase1 and NTPDase2 after heterologous expression in Xenopus oocytes. It may also be used as an esterase inhibitor, which is added to the whole blood to improve the stability of ciclesonide for the determination of ciclesonide (CIC) and its active metabolite, desisobutyryl-ciclesonide (des-CIC), in human plasma using liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometric (LC-APCI-MS/MS) method.

Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.

Recommended concentrations for use are 0.1-1 mM.

생화학적/생리학적 작용

4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) is an irreversible serine protease inhibitor. Inhibition constants are similar to those of phenylmethanesulfonyl fluoride (PMSF) and diisopropylfluorophosphate (DFP). AEBSF has been shown to inhibit trypsin, chymotrypsin, plasmin kallikrein, and thrombin. As an alternative to PMSF and DFP, AEBSF offers lower toxicity, improved solubility in water, and improved stability in aqueous solutions. AEBSF can be used in cell culture in concentrations of up to 0.25 mM.

기타 정보

Potent and irreversible inhibitor of serine proteases; in contrast to PMSF, it is water soluble and non-toxic; Unaffected by albumin

법적 정보

Pefabloc is a registered trademark of Pentapharm

안전성 정보

• 픽토그램: GHS05
• 신호어: Danger
• 유해 및 위험 성명서: H314
• 예방조치 성명서: P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363
• Hazard Classifications: Eye Dam. 1 - Skin Corr. 1A
• Storage Class Code: 8A - Combustible corrosive hazardous materials
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges