90922

Mueller Hinton Broth 2

NutriSelect^® Plus, suitable for microbiology, Cation-adjusted

• 동의어(들): M-H 2 Broth, M-H 2 Broth, Mueller Hinton II Broth, Mueller Hinton II Broth
• UNSPSC 코드: 41171504
• NACRES: NA.85

속성

• Quality Level: 200
• 무균: non-sterile
• 형태: powder
• 유통기한: limited shelf life, expiry date on the label
• 구성: acid hydrolysate of casein, 17.5 g/L ; beef extract, 3 g/L ; starch, 1.5 g/L
• 포장: bottle of 500 g
• 제조업체/상표: NutriSelect^® Plus
• 기술: microbe id | susceptibility testing: suitable; microbiological culture: suitable
• 색상: light yellow
• 최종 pH: 7.3±0.2 (25 °C)
• 응용 분야: clinical testing; food and beverages; microbiology
• 적합성: nonselective for Enterococcus spp.; nonselective for Escherichia coli; nonselective for Neisseria spp.; nonselective for Pseudomonas spp.; nonselective for Staphylococcus spp.; nonselective for bacteria (General Media); nonselective for coliforms

설명

일반 설명

Mueller Hinton Broth 2 is recommended for testing the most encountered aerobic and facultative anaerobic bacteria found in food and clinical material for antimicrobial susceptibility testing. The medium shows good batch-to-batch reproducibility. It is low in sulfonamide, trimethoprim, and tetracycline inhibitors and yields satisfactory growth of most non-fastidious pathogens.Casein acid hydrolysate and beef extract provide amino acids and other nitrogenous substances, minerals, vitamins, and carbon to support the growth of microorganisms. To determine the MIC values with Enterococcus faecalis and sulfamethoxazole-trimethoprim, a special low content of thymine and thymidine in the amino acid sources is required. Starch neutralizes toxic substances that may be present in the medium and have an inhibitory effect on antibiotics and the growth of organisms. During autoclaving, a small amount of dextrose is hydrolyzed from starch, which is a source of energy. Calcium and magnesium salts are added to adjust the ion concentrations of the medium.

애플리케이션

Mueller Hinton Broth 2 cation adjusted is intended for use in quantitative procedures for susceptibility testing of rapidly growing aerobic and facultatively anaerobic bacteria isolated from clinical specimens. It has been used for culturing Escherichia coli, Bacillus subtilis, Staphylococcus aureus and in the serial dilution of doxycycline.

특징 및 장점

NutriSelect^® Plus are powdered culture media offering

• Quality control with growth promotion test according to industry standards
• Quality assurance acc. to ISO 9001
• Cost-efficient powdered media

제조 메모

Suspend 22 g in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Dispense and sterilize by autoclaving at 10-15 lbs pressure (115-121°C) for 10 minutes. DO NOT OVERHEAT. Mix well before pouring.
Note: This medium is supplemented with appropriate salts to provide 20-25 mg/l of calcium and 10-12.5 mg/l of magnesium and as additionally required to suit performance parameters.

각주

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

법적 정보

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)