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Merck
모든 사진(3)

주요 문서

A7174

Sigma-Aldrich

Agarose

High Gelling Temperature

동의어(들):

3,6-Anhydro-α-L-galacto-β-D-galactan

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC 코드:
41105317
PubChem Substance ID:
NACRES:
NA.25

Quality Level

유형

Type VI-A

형태

powder

불순물

≤10% water

≤0.60%

EEO

≤0.14

전이 온도

gel point 41 °C ±1.5 °C (1.5% gel)

겔 강도

≥900 g/cm2 (1% gel)

음이온 미량물

sulfate (SO42-): ≤0.20%

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

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애플리케이션


  • Quality and applicability of cadaveric donor eyes for molecular biology research: An Indian experience.: This study discusses the use of agarose in preparing donor eyes for molecular biology research, highlighting its effectiveness in preserving samples for high-quality genetic analysis (Kaur et al., 2024).

  • Immunocytochemistry on frozen-embedded cell block for the diagnosis of hematolymphoid cytology specimen: a straightforward alternative to the conventional cell block.: Agarose is used to enhance the immunocytochemistry process, providing a more efficient and clear diagnostic method for hematolymphoid diseases (Choi et al., 2024).

  • Low-melting point agarose as embedding medium for MALDI mass spectrometry imaging and laser-capture microdissection-based proteomics.: Demonstrates the use of low-melting point agarose as a versatile medium for advanced imaging and proteomic analysis, contributing significantly to analytical biochemistry (McDonnell et al., 2023).

  • Autocrine regulation of tumor cell repopulation by Hsp70-HMGB1 alarmin complex.: Agarose is utilized in the study of cellular mechanisms for cancer research, underscoring its importance in experimental setups requiring precise biological simulations (Guzhova et al., 2023).

분석 메모

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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An integrated approach to temperature-controlled chromatography, involving copolymer modified agarose adsorbents and a novel travelling cooling zone reactor (TCZR) arrangement, is described. Sepharose CL6B was transformed into a thermoresponsive cation exchange adsorbent (thermoCEX) in four synthetic steps: (i) epichlorohydrin activation;
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